The related adhesion focal tyrosine kinase forms a complex with paxillin in hematopoietic cells.

The Journal of Biological Chemistry
R SalgiaJ D Griffin

Abstract

Related adhesion focal tyrosine kinase (RAFTK), also known as proline-rich tyrosine kinase 2 and cellular adhesion kinase beta, has been recently cloned and characterized as a member of the focal adhesion kinase (FAK) subfamily. RAFTK has an overall 48% amino acid homology to p125(FAK) and contains a kinase domain but lacks a transmembrane region, myristylation sites, and Src homology region 2 and 3 domains. By Northern blot analysis, RAFTK is expressed in myeloid, lymphoid, and megakaryocytic hematopoietic cells. Like p125(FAK), we found that RAFTK interacts with the focal adhesion protein paxillin. In the lymphoid cell line BaF3 and the myeloid cell line 32Dcl3, RAFTK coprecipitates with paxillin. Using in vitro binding assays, RAFTK and paxillin were shown to bind directly, through a segment of paxillin that required amino acids 100-227 and a domain in the C terminus of RAFTK. In vitro, RAFTK could phosphorylate paxillin on tyrosine residues. These results suggest that RAFTK, as well as p125(FAK), may be important in phosphotyrosine-signaling events within the focal adhesion.

References

Nov 6, 1992·Science·E J Luna, A L Hitt
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M D SchallerJ T Parsons
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·B GeigerT Volberg
Dec 8, 1995·The Journal of Biological Chemistry·R SalgiaL B Chen
Mar 1, 1994·Molecular and Cellular Biology·M D SchallerJ T Parsons
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·S B KannerP Y Chan
Mar 10, 1995·The Journal of Biological Chemistry·R SalgiaT Ernst
May 1, 1995·Molecular and Cellular Biology·M D Schaller, J T Parsons
Oct 1, 1995·The Journal of Experimental Medicine·K TachibanaC Morimoto
Nov 1, 1995·The Journal of Cell Biology·S MiyamotoK M Yamada
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·S KharbandaD Kufe
Jul 21, 1995·The Journal of Biological Chemistry·S L BellisC E Turner
Oct 1, 1994·Current Opinion in Cell Biology·M D Schaller, J T Parsons
Jan 1, 1994·Journal of Cell Science. Supplement·J T ParsonsC Otey
Mar 1, 1994·Cancer Metastasis Reviews·S H Lo, L B Chen

❮ Previous
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Citations

Feb 1, 2002·Biochemical and Biophysical Research Communications·Rosemary J KeoghCaroline P D Wheeler-Jones
May 3, 2011·Clinical & Experimental Metastasis·Zhihong ZhangKathryn E Meier
Jun 4, 1999·Progress in Biophysics and Molecular Biology·D D SchlaepferD J Sieg
Mar 7, 2001·Biology of the Cell·V Petit, J P Thiery
Jun 23, 1999·Experimental Hematology·S GhaffariC Eaves
Oct 26, 1999·Cellular Signalling·G Berton, C A Lowell
Mar 8, 2000·Cellular Signalling·H AvrahamS Avraham
Aug 12, 1998·Nature Structural Biology·M C BrownC E Turner
Aug 8, 2012·The Journal of Biological Chemistry·Amy L StieglerTitus J Boggon
Oct 31, 2003·Molecular and Cellular Biology·Yoshihiro TaniyamaKathy K Griendling
Jan 1, 1997·Annual Review of Cell and Developmental Biology·S M Thomas, J S Brugge
Jun 13, 2009·American Journal of Respiratory Cell and Molecular Biology·Yingli DuanXiangdong Zhu
Apr 1, 1997·The Journal of Clinical Investigation·Z Y LiuJ E Groopman
Jul 1, 1997·The Journal of Clinical Investigation·R SalgiaJ D Griffin
Aug 3, 1999·The Journal of Clinical Investigation·M FuortesC Nathan
Apr 9, 2002·Cell Structure and Function·Hiroshi AotoTerukatsu Sasaki
Dec 9, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M JeschkeM Susa
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·M OkigakiJ Schlessinger
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J S FelschE G Peralta
Mar 14, 2003·Seminars in Oncology·Martin Sattler, Ravi Salgia
Dec 24, 1997·Leukemia & Lymphoma·S Avraham, H Avraham
Apr 18, 2000·The Journal of Cell Biology·H UedaJ L Guan
May 6, 1999·Cell Adhesion and Communication·I YronA Altman
Sep 2, 2014·Journal of Molecular Biology·Murugendra S VanarottiJie J Zheng
Nov 4, 2009·Journal of the American Society of Hypertension : JASH·Fernanda R C GiachiniRita C Tostes
Aug 30, 2003·Biological Psychiatry·Teige P SheehanDavid S Russell
Jul 28, 2006·Biochemical Pharmacology·Christiane Kummer, Mark H Ginsberg
Sep 14, 2014·Blood·Yu ZhangIrene M Ghobrial

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