PMID: 39969Aug 1, 1979

The relation between dicarbocyanine dye fluorescence and the membrane potential of human red blood cells set at varying Donnan equilibria

The Journal of General Physiology
J C Freedman, J F Hoffman

Abstract

The fluorescence, F, of two dicarbocyanine dyes, diS-C3(5) and diI-C3(5), depends both on the membrane potential, E, and on the intracellular pH, pHc, or human red blood cells. Compositions of isotonic media have been devised in which the equilibrium Donnan potential, E, varies at constant pHc and in which pHc varies at constant E. Dye fluorescence measurements in these suspensions yield calibrations of +1.7 % delta F/mV for diS-C3(5) and +0.6 % delta F/mV for diI-C3 (5). While pHo does not affect F of either dye, changes in pHc of 0.1 unit at constant E cause changes of F equivalent to those induced by 2--3mV. Based on these results, a method is given for estimating changes in E from dye fluorescence in experiments in which E and pHc co-vary. The relation of F to E also depends in a complex way on the type and concentration of cells and dye, and the wavelengths employed. The equilibrium calibration of dye fluorescence, when applied to diffusion potentials induced by 1 microM valinomycin, yields a value for the permeability ratio, PK.VAL/PCl, of 20 +/- 5, in agreement with previous estimates by other methods. The calibration of F is identical both for diffusion potentials and for equilibrium potentials, implying that diC-C3(5) ...Continue Reading

Citations

Jan 1, 1984·The Journal of Membrane Biology·E M BifanoJ C Freedman
Jan 1, 1987·The Journal of Membrane Biology·G R Kracke, P B Dunham
Jul 20, 2012·Journal of Bioenergetics and Biomembranes·J PlásekMilan Höfer
Aug 1, 1995·Progress in Neurobiology·T J Ebner, G Chen
Apr 1, 1996·Journal of Photochemistry and Photobiology. B, Biology·J Plásek, K Sigler
Nov 1, 1981·European Journal of Biochemistry·W C HorneE R Simons
Feb 20, 2007·Infection and Immunity·Carina GonçalvesJ Daniel Dubreuil
Apr 30, 2014·Annual Review of Biophysics·Adam E Cohen, Veena Venkatachalam
Jan 1, 1989·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·R R Gonzalez-Mendez, G M Hahn
Mar 17, 2010·Blood Cells, Molecules & Diseases·Edyta GlogowskaSerge L Y Thomas
Oct 8, 2017·European Biophysics Journal : EBJ·Saša Svetina
Aug 1, 1982·Journal of Cellular Physiology·T C Smith
Mar 1, 1991·Archives of Andrology·L CalzadaH Macias

Related Concepts

Cell Membrane Permeability
Diffusion
Erythrocytes
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