The relationship between compositional phase separation and vesicle morphology: implications for the regulation of phospholipase A2 by membrane structure

Chemistry and Physics of Lipids
W R BurackR L Biltonen

Abstract

The action of phospholipase A2 (PLA2) on bilayer substrates causes the accumulation of reaction products, lyso-phospholipid and fatty acid. These reaction products and the phospholipid substrate generate compositional heterogeneities and then apparently phase separate when a critical mole fraction of reaction product accumulates in the membrane. This putative phase separation drives an abrupt morphologic rearrangement of the vesicle, which may be in turn responsible for modulating the activity of PLA2. Here we examine the thermotropic properties of the phase-separated lipid system formed upon hydrating colyophilized reaction products (1:1 palmitic acid:1-palmitoyl-2-lyso-phosphatidylcholine) and substrate, dipalmitoylphosphatidylcholine. The mixture forms structures which are not canonical spherical vesicles and appear to be disks in the gel-state. The main gel-liquid transition of these structures is hysteretic. This hysteresis is apparent using several techniques, each selected for its sensitivity to different aspects of a lipid aggregate's structure. The thermotropic hysteresis reflects the coupling between phase separation and changes in vesicle morphology.

References

Dec 20, 1979·Biochimica Et Biophysica Acta·P R Cullis, B de Kruijff
Mar 1, 1991·Chemistry and Physics of Lipids·W KnollJ Spinke
Jul 27, 1990·Cell·K Simons, A Wandinger-Ness
Jan 8, 1980·Biochemistry·T N EstepT E Thompson
Sep 6, 1994·Chemistry and Physics of Lipids·W R Burack, R L Biltonen
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·O G Mouritsen, M Bloom
Jun 5, 1997·Nature·K Simons, E Ikonen

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Citations

Sep 25, 2002·Biochemical and Biophysical Research Communications·Lifeng CaiLuhua Lai
Apr 19, 2015·Advances in Colloid and Interface Science·Srinivasan Damodaran
Feb 8, 2002·Archives of Biochemistry and Biophysics·Rogelio Morales, Marta S Fernández

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