The relationship between curvature, flexibility and persistence length in the tropomyosin coiled-coil.

Journal of Structural Biology
Xiaochuan Edward LiStefan Fischer

Abstract

The inherent flexibility of rod-like tropomyosin coiled-coils is a significant factor that constrains tropomyosin's complex positional dynamics on actin filaments. Flexibility of elongated straight molecules typically is assessed by persistence length, a measure of lengthwise thermal bending fluctuations. However, if a molecule's equilibrium conformation is curved, this formulation yields an "apparent" persistence length ( approximately 100nm for tropomyosin), measuring deviations from idealized straight conformations which then overestimate actual dynamic flexibility. To obtain the "dynamic" persistence length, a true measurement of flexural stiffness, the average curvature of the molecule must be taken into account. Different methods used in our studies for measuring the dynamic persistence length directly from Molecular Dynamics (MD) simulations of tropomyosin are described here in detail. The dynamic persistence length found, 460+/-40nm, is approximately 12-times longer than tropomyosin and 5-times the apparent persistence length, showing that tropomyosin is considerably stiffer than previously thought. The longitudinal twisting behavior of tropomyosin during MD shows that the amplitude of end-to-end twisting fluctuation is...Continue Reading

References

May 1, 1989·Biopolymers·C A Swenson, N C Stellwagen
Oct 1, 1984·Biophysical Journal·J S Shiner, R J Solaro
Oct 1, 1980·Biophysical Journal·G N PhillipsC Cohen
Jan 5, 1981·Journal of Molecular Biology·C ChothiaD Richardson
Jan 1, 1996·Annual Review of Physiology·L S Tobacman
Feb 14, 1997·Journal of Molecular Biology·P VibertW Lehman
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·J H BrownC Cohen
Mar 20, 2002·Biophysical Journal·M A Geeves, S S Lehrer
Dec 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jerry H BrownCarolyn Cohen
Aug 8, 2006·Biophysical Journal·Jory Z Ruscio, Alexey Onufriev
Feb 7, 2007·Physical Review Letters·Charles W Wolgemuth, Sean X Sun
Jan 1, 2008·The Journal of Biological Chemistry·John P SumidaSherwin S Lehrer
Jan 1, 2009·Journal of Muscle Research and Cell Motility·Kenneth C Holmes, William Lehman
May 16, 2009·Journal of Computational Chemistry·B R BrooksM Karplus
Nov 4, 2009·Journal of Molecular Biology·Xiaochuan Edward LiStefan Fischer

❮ Previous
Next ❯

Citations

Oct 12, 2012·European Biophysics Journal : EBJ·Srboljub M MijailovichMichael A Geeves
Feb 12, 2011·PloS One·Tara D SutherlandSarah Weisman
Jan 23, 2016·Structural Dynamics·Stefan FischerWilliam Lehman
Jul 14, 2012·Biochemical and Biophysical Research Communications·Xiaochuan Edward LiWilliam Lehman
Nov 1, 2011·Journal of Molecular Biology·Anthony CammaratoSanford I Bernstein
Nov 21, 2015·Biophysical Journal·Yasser AboelkassemStuart G Campbell
Oct 31, 2014·Molecular Microbiology·Narges MortezaeiEsther Bullitt
Sep 12, 2013·Journal of Molecular Biology·Duncan R SousaM Elizabeth Stroupe
Nov 2, 2011·Journal of Molecular Biology·Yusuke OguchiMasataka Kawai
Dec 22, 2010·Cell Adhesion & Migration·Justin G LeesGeraldine M O'Neill
Jul 24, 2012·Cell·Elmar BehrmannStefan Raunser
Aug 27, 2013·Biophysical Journal·Nadia A Metalnikova, Andrey K Tsaturyan
Mar 13, 2014·Biochemical and Biophysical Research Communications·Xiaochuan Edward LiStefan Fischer
Sep 25, 2016·Archives of Biochemistry and Biophysics·Michael J RynkiewiczWilliam Lehman
Nov 12, 2016·Frontiers in Physiology·Lorenzo R SewananStuart G Campbell
Dec 14, 2016·Archives of Biochemistry and Biophysics·Yurii S BorovikovJoanna Moraczewska
Dec 27, 2017·The FEBS Journal·Alexander M MatyushenkoDmitrii I Levitsky
Mar 13, 2018·The Journal of Infectious Diseases·Jeffrey W BrownEsther Bullitt
Dec 3, 2017·Scientific Reports·Yurii S BorovikovCharles S Redwood
Feb 17, 2019·The Journal of Physiology·William Schmidt, Anthony Cammarato
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·I A Nevzorov, D I Levitsky
Apr 6, 2019·The Journal of General Physiology·Srboljub M MijailovichHenk L Granzier
May 26, 2018·The Journal of Cell Biology·J Richard McIntoshNikita Gudimchuk
Jul 22, 2020·Proceedings of the National Academy of Sciences of the United States of America·Aditi MadanAnthony Cammarato
Oct 18, 2020·International Journal of Molecular Sciences·Olga E KarpichevaYurii S Borovikov
Nov 22, 2020·International Journal of Molecular Sciences·Marina A MarchenkoAlexander M Matyushenko
Oct 14, 2019·Current Opinion in Chemical Biology·Michael Wh KirknessNancy R Forde
May 23, 2021·Biophysical Journal·Mathew SchneiderNancy R Forde
Jul 29, 2021·The Journal of General Physiology·Lorenzo R SewananStuart G Campbell
Apr 4, 2012·The Journal of Organic Chemistry·Pablo Wessig, Kristian Möllnitz
Sep 27, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Taraknath Mandal, Ronald G Larson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.