The relationship between metastatic potential and in vitro mechanical properties of osteosarcoma cells

Molecular Biology of the Cell
Claude N HolensteinUnai Silvan

Abstract

Osteosarcoma is the most frequent primary tumor of bone and is characterized by its high tendency to metastasize in lungs. Although treatment in cases of early diagnosis results in a 5-yr survival rate of nearly 60%, the prognosis for patients with secondary lesions at diagnosis is poor, and their 5-yr survival rate remains below 30%. In the present work, we have used a number of analytical methods to investigate the impact of increased metastatic potential on the biophysical properties and force generation of osteosarcoma cells. With that aim, we used two paired osteosarcoma cell lines, with each one comprising a parental line with low metastatic potential and its experimentally selected, highly metastatic form. Mechanical characterization was performed by means of atomic force microscopy, tensile biaxial deformation, and real-time deformability, and cell traction was measured using two-dimensional and micropost-based traction force microscopy. Our results reveal that the low metastatic osteosarcoma cells display larger spreading sizes and generate higher forces than the experimentally selected, highly malignant variants. In turn, the outcome of cell stiffness measurements strongly depends on the method used and the state of t...Continue Reading

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Citations

Oct 18, 2020·International Journal of Molecular Sciences·Anita K Luu, Alicia M Viloria-Petit
Nov 26, 2020·Developmental Cell·Valentin GensbittelJacky G Goetz
Mar 2, 2021·Frontiers in Bioengineering and Biotechnology·Sebastian E Amos, Yu Suk Choi
Apr 23, 2021·ACS Applied Materials & Interfaces·Ajinkya GhagreAllen Ehrlicher
May 1, 2021·Cells·Gaël RunelIngrid Masse
Jun 9, 2021·Biomaterials·Charlotte AlibertJean-Baptiste Manneville

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Methods Mentioned

BETA
atomic force microscopy
flow cytometry
chip

Software Mentioned

JPK Data Processing
UCSF Chimera
bUnwarpJ
ImageJ
CellSegm
Matlab
ShapeOut

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