Abstract
Radiofrequency (RF) ablation is a nonsurgical technique using catheter-directed RF energy for treating cardiac arrhythmias in children and adults. Previous reports have suggested that sequestration of calcium (Ca2+) by the sarcoplasmic reticulum may partially protect mature cardiac myocytes from the effects of RF energy. The purposes of this study were to determine whether differences exist between neonatal and adult myocyte responses to RF energy and if myocyte damage is a Ca2+-dependent process. Because immature myocardium is functionally deficient in sarcoplasmic reticulum, we hypothesized that immature myocytes would be more susceptible to damage induced by RF energy. Isolated ventricular myocytes were obtained from neonatal and adult New Zealand White rabbits by enzymatic dissociation, then placed in a perfusion chamber designed to deliver RF energy or a heated perfusate solution. Measurements of bath temperature, cell morphology, and contractile response to electrical stimuli were recorded. RF energy application associated with increased perfusate temperature resulted in cell death, but not when the temperature rise was inhibited. Thus, the acute damage to cells exposed to RF energy appears to be mediated by thermal energ...Continue Reading
References
Mar 1, 1992·The American Journal of Physiology·M AraiM Periasamy
Nov 1, 1992·Circulation·J J LangbergF Morady
Jun 1, 1992·Journal of the American College of Cardiology·J J LangbergF Morady
May 1, 1991·Pacing and Clinical Electrophysiology : PACE·L T BlouinL Lampe
Nov 1, 1991·Pacing and Clinical Electrophysiology : PACE·O Tovar, L Tung
Nov 1, 1991·Circulation Research·A M LompréK Schwartz
May 1, 1991·Biophysical Journal·R J O'Neill, L Tung
Sep 1, 1990·Circulation·D E Haines, A F Verow
Jan 1, 1989·Pacing and Clinical Electrophysiology : PACE·W HaverkampG Breithardt
Jun 1, 1989·Pacing and Clinical Electrophysiology : PACE·D E Haines, D D Watson
Nov 1, 1989·European Heart Journal·G HindricksG Breithardt
Apr 1, 1986·Circulation·J H LevineE N Moore
Dec 1, 1984·Neurosurgery·E R CosmanJ Ovelman-Levitt
Feb 1, 1980·Journal of Biomechanical Engineering·A Erez, A Shitzer
Nov 1, 1993·Pacing and Clinical Electrophysiology : PACE·M DickB Armstrong
Mar 1, 1993·Pacing and Clinical Electrophysiology : PACE·D E Haines
Jul 1, 1994·Circulation·C Lenfant
Jul 1, 1994·Circulation·J P SaulE P Walsh
Sep 1, 1994·Circulation·H CalkinsP Gillette
Jul 1, 1993·Circulation·S D Ballin
Oct 1, 1993·Circulation·S NathD E Haines
Citations
Mar 23, 2005·Neuroscience·O Cruz, D P Kuffler
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Jennifer M GordonRebecca H Ritchie
Feb 28, 2008·Radiation Research·V S RaoM R Cho