The relationship of HsEg5 and the actin cytoskeleton to centrosome separation

Cell Motility and the Cytoskeleton
C M WhiteheadJ B Rattner

Abstract

Although centrosome separation is essential to the formation of a bipolar spindle, it can proceed along several different pathways. This raises questions as to the similarity between the mechanism(s) underlying these various forms of separation. To address this question we reinvestigated centrosome separation in HeLa cells using a variety of techniques. We present a refined description of the two major pathways of centrosome separation found in HeLa cells and demonstrate that each of these pathways has its own timing, protein requirements, morphological characteristics, and relationship to spindle assembly. The first pathway, which occurs in prophase cells, is dependent on an intact actin cytoskeleton, and when this pathway is completed prior to nuclear envelope breakdown, the microtubules associated with this process do not become part of the spindle. Thus, centrosome separation and spindle pole organization can occur as two separate events. The second centrosome separation pathway is found in cells in which separation occurs concurrent with prometaphase. In this case, centrosome separation and the formation of the mitotic spindle are integrated together and an intact actin cytoskeleton is not required. The relationship betwee...Continue Reading

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Citations

Apr 29, 2005·Molecular Biology of the Cell·Daniela A BritoMichael P Koonce
Apr 5, 2012·The Journal of Cell Biology·Balca R Mardin, Elmar Schiebel
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