The relationships among stem cells, crypts, and villi in the small intestine of mice as determined by mutation tagging

Developmental Dynamics : an Official Publication of the American Association of Anatomists
L CosentinoJ A Heddle

Abstract

The number of stem cells that maintain a crypt of the small intestine is uncertain. Although the number of stem cells per crypt had been thought to be 10-20, current estimates indicate a much smaller number, possibly 1 stem cell per crypt. We report here that in SWR and C57BL/6 X SWR F1 mice, the results of mutation tagging are inconsistent with the existence of more than one stem cell per crypt. Mutations at the Dlb-1 locus mark the progeny of a single epithelial stem cell in the small intestine and reveal the size of the mutant clone. The epithelial cells are produced in the crypts and migrate up the villi to be sloughed off at the tip so that a mutant clone takes the form of a ribbon on the villus. Because the size of the mutant clones must be inversely proportional to the number of stem cells contributing to the villus, this provides a means of counting the stem cells (ethylnitrosourea was used to induce mutations in the intestine). In the duodenum the ribbons average 0.099 +/- 0.004 villus circumferences, indicating that there are 1/0.099 = 10.2 stem cells per villus. Because there are 10.5 +/- 0.67 crypts/villus, the estimate gives 10.2/10.5 = 0.97 +/- 0.1 stem cells/crypt. Because each crypt must have at least one stem c...Continue Reading

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Citations

Dec 8, 1998·Pathology, Research and Practice·S E KongJ Hall
Apr 12, 2008·Molecular and Cellular Endocrinology·Katherine J Rowland, Patricia L Brubaker
Sep 29, 2004·Mutation Research·Yanping Wang, John A Heddle
Nov 16, 2005·Gastroenterology·Christopher M DekaneySusan J Henning
Apr 25, 2016·The Journal of Physiology·Susan J Henning, Richard J von Furstenberg
Mar 23, 2012·Animal : an International Journal of Animal Bioscience·A V CapucoC M Evock-Clover
Jan 1, 2013·Annual Review of Animal Biosciences·Anthony V Capuco, Steven E Ellis
Jan 1, 1996·Environmental and Molecular Mutagenesis·J A HeddleY Paashuis-Lew
Jan 1, 1996·Environmental and Molecular Mutagenesis·L Cosentino, J A Heddle
Jan 30, 2009·Experimental Biology and Medicine·Anthony V CapucoDavid L Wood
Dec 27, 2008·Bulletin of Experimental Biology and Medicine·L A VedinaV A Kozlov
Dec 3, 2002·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Shunzo Kobayashi
May 12, 2005·Journal of Mammary Gland Biology and Neoplasia·A V Capuco, S Ellis
Feb 13, 2001·Microscopy Research and Technique·G H Smith, G Chepko
Oct 18, 2000·Mutation Research·L Cosentino, J A Heddle

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