PMID: 6975713Jan 1, 1980Paper

The relative contributions of B and T lymphocytes in the human peripheral blood mutagen test system as determined by cell survival, mitogenic stimulation, and induction of chromosome aberrations by radiation

Environmental Mutagenesis
J L Schwartz, M E Gaulden

Abstract

Ficoll-Hypaque-separated subpopulations of human peripheral blood T and B lymphocytes were exposed to 0, 0.5, 1.0, 2.5, or 5.0 Gy of gamma rays. Three parameters were examined: Survival, as measured by trypan blue dye exclusion in unstimulated cultures five days after irradiation; mitotic index, measured in phytohemagglutinin (PHA)-stimulated cultures 48 and 72 hours after irradiation; and chromosome aberration frequency, measured 48 or 60 hours after irradiation. Survival curves of T, B, and null cells are biphasic; the Do values for the radiosensitive populations of all three cell types are close to 0.6 Gy but are different for the radioresistant populations: 2.7 Gy for B cells, 4.77 Gy for T cells, and 6.03 Gy for null cells. B cells, as well as T cells, are stimulated to divide by PHA, and B cells comprise at least 10% of the mitotic figures seen in unirradiated cultures at 48 hours. The proportion of B lymphocytes in mitosis at any particular time after PHA stimulation decreases with increasing radiation dose, which reflects a higher mitotic radiosensitivity of B than of T cells. No significant difference, however, in chromosome aberration frequency was found between T and B cells.

References

Jan 1, 1979·Immunological Reviews·H CantorL Glimcher
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Feb 23, 1978·Human Genetics·P E Crossen, W F Morgan
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Citations

May 1, 1985·Mutation Research·R T DutkowskiM E Weksler
Apr 1, 1993·Mutation Research·K WuttkeW U Müller
Jan 1, 1991·Pharmacology & Therapeutics·M C EliaM O Bradley
Dec 7, 2002·The International Journal of Biochemistry & Cell Biology·Aya KurosawaHirobumi Teraoka
May 16, 2014·Radiation Research·Marina V BakhmutskyJames D Tucker

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