PMID: 8961165Nov 1, 1996Paper

The relaxant effect of extract of Phyllanthus urinaria in the guinea-pig isolated trachea. Evidence for involvement of ATP-sensitive potassium channels

The Journal of Pharmacy and Pharmacology
N PaulinoJ B Calixto

Abstract

This study analyses the relaxation induced by the hydroalcoholic extract of stems, leaves and roots from Phyllanthus urinaria (Euphorbiaceae) in the guinea-pig trachea (GPT) pre-contracted by carbachol. The hydroalcoholic extract of P. urinaria (0.1-10 mg mL-1) caused a graded relaxation in GPT with or without epithelium, with mean EC50 values of 1.94 (1.41-2.67) and 2.00 (1.47-2.78) mg mL-1 and Emax of 717 mg (+/- 16) and 627 mg (+/- 12), respectively. The relaxation in response to hydroalcoholic extract, like that to cromakalim (EC50 3.57 (2.75-4.64 microM) in GPT without epithelium, was fully abolished in the presence of high KCl concentrations (80 mM), and was significantly attenuated by tetraethylammonium (10 or 30 mM) or glibenclamide (0.1 or 3 microM). However, the relaxation caused by the hydroalcoholic extract was unaffected by apamin (0.1 or 1.0 microM), nitro-L-arginine (L-NOARG, 100 microM), methylene blue (10 microM) or by calcitonin gene-related peptide (CGRP) (8-37) (a CGRP antagonist, 0.1 microM). Both propranolol (1 or 3 microM) and [D-p-Cl-Phe6,Leu17]VIP (a vasoactive intestinal peptide (VIP) receptor antagonist, 0.1 microM) produced a significant displacement to the right (about 2-fold) of the relaxation resp...Continue Reading

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Citations

Aug 10, 2006·Urological Research·Marcio E BarrosMirian A Boim
Feb 13, 2001·Journal of Ethnopharmacology·M C MarcucciN Paulino
Oct 8, 2009·Evidence-based Complementary and Alternative Medicine : ECAM·Hua-Yew ChengChun-Ching Lin
Jan 27, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Rafael C DutraJoão B Calixto
Apr 29, 2006·International Immunopharmacology·Sheng-Teng HuangJong-Hwei S Pang
Jun 11, 2015·Phytotherapy Research : PTR·Arif-Ullah Khan, Anwarul-Hassan Gilani
Jun 25, 2002·The Journal of Pharmacy and Pharmacology·Niraldo PaulinoJoão B Calixto
Jul 31, 2007·Phytotherapy Research : PTR·Chien-Min YangChun-Ching Lin
Jul 4, 2006·The Journal of Urology·Serhat Gürocak, Bora Küpeli
Jan 20, 2011·BJU International·Shrawan K SinghSumit Sharma
Oct 18, 2018·Frontiers in Pharmacology·Madamanchi Geethangili, Shih-Torng Ding
Aug 29, 2000·Phytotherapy Research : PTR·J B CalixtoR A Yunes

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