PMID: 6113268May 1, 1981Paper

The release of gamma-aminobutyric acid, glutamate, and acetylcholine from striatal slices: a mass fragmentographic study

Journal of Neurochemistry
F MoroniL Beani

Abstract

The release processes of endogenous Acetylcholine (ACh), gamma-aminobutyric acid (GABA), glutamate (Glu) and glutamine (GLN) were studied in superfused guinea-pig caudatal slices. Basal ACh release remained constant for up to 2 h, while the basal release of GABA, Glu and GLN declined to half or less of its initial values after 1 h of superfusion. Electrical stimulation increased the ACh release by 700-800% and that of GABA by 80% whereas it decreased the output of Glu by 50% and failed to modify the GLN efflux. KCl (25 nM) increased the output of ACh by 400%, that of GABA by approximately 500% and decreased that of Glu by 40%. Substituting of CaCl(2) by MgCl(2) in the superfusion medium reduced the basal efflux of GABA, Glu and GLN. Under these conditions, no evoked release of ACh or of GABA was detected, following electrical or KCl stimulation. Tetrodotoxin 5 x 10(-7) decreased the basal ACh release by 60% and increased the GABA efflux by 40%. The toxin abolished the stimulus-evoked ACh efflux but scarcely affected that of GABA. These results are consistent with a possible neurotransmitter role of ACh and GABA in the striatum and show some differences in the ionic mechanisms underlying GABA and ACh release.

References

Jan 1, 1979·Neuroscience·G E Fagg, J D Lane
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C W ChristopherH M Kalckar
Oct 1, 1979·European Journal of Pharmacology·C BianchiL Beani
Mar 15, 1978·European Journal of Pharmacology·L BeaniF Tamberi
Oct 1, 1974·Physiological Reviews·T Narahashi
Jun 1, 1969·Journal of Neurochemistry·R I KatzI J Kopin
May 1, 1967·Journal of Neurochemistry·Y MachiyamaD Richter
Apr 1, 1963·The Journal of Pharmacy and Pharmacology·L BEANI, C BIANCHI

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Citations

Jan 1, 1989·Neurochemistry International·S BernathM J Zigmond
Apr 9, 2017·Applied and Environmental Microbiology·Amanda HeJoan L Slonczewski
Dec 1, 1988·Journal of Neurochemistry·D E Pellegrini-GiampietroF Moroni
Sep 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·F MoroniF Fiaccadori

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