PMID: 8584013Oct 4, 1995Paper

The release of glutamate and aspartate from rat brain synaptosomes in response to domoic acid (amnesic shellfish toxin) and kainic acid

Molecular and Cellular Biochemistry
J A Brown, M S Nijjar

Abstract

Kainic acid is known to stimulate the release of glutamate (GLU) and aspartate (ASP) from presynaptic neurons. It has been suggested that the enhanced release of these endogenous EAA's plays a significant role in the excitotoxic effects of KA. Domoic acid (DOM), a shellfish toxin, is structurally similar to KA, and has been shown to be 3-8 times more toxic than KA. In this study, effects of KA and DOM on the release of GLU and ASP from rat brain synaptosomes were investigated. Amino acid analysis was performed by the reversed phase HPLC, following derivatization with 9-fluorenylmethyl chloroformate (FMOC). Potassium chloride (40 mM) was used as a positive control, and stimulated GLU release from rat brain synaptosomes in presence or absence of Ca2+. DOM enhanced the release of GLU, whereas KA stimulated the release of both GLU and ASP from synaptosomes in the presence of Ca2+. However, their potency to stimulate GLU and ASP release was enhanced in absence of Ca2+. These results indicate that different mechanisms may be involved in the release of GLU and ASP in response to DOM and KA, and that neurotransmitter release appeared to be highly specific for these agonists. It would appear that DOM and KA may interact with different r...Continue Reading

References

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Citations

Aug 25, 2004·Neurotoxicity Research·Ananth ChandrasekaranCharanjit Kaur
Aug 10, 2013·Analytical and Bioanalytical Chemistry·Xiangtang LiYi-Ming Liu
Jul 20, 2006·Bulletin of Experimental Biology and Medicine·I V ErmakovaJ Sinden
Aug 5, 2000·European Journal of Pharmacology·B A QuintelaM Alfonso
Apr 18, 2001·Toxicon : Official Journal of the International Society on Toxinology·Z AmzilC Billard
May 23, 2001·Progress in Neurobiology·N C Danbolt
Jul 4, 2001·Neurochemistry International·M W FleckA M Palmer
Nov 28, 2009·Toxicon : Official Journal of the International Society on Toxinology·Nicolas RuizYves François Pouchus
Jul 4, 2006·Clinics in Laboratory Medicine·Matthew SalzmanMichael I Greenberg
May 25, 2012·Pain Medicine : the Official Journal of the American Academy of Pain Medicine·Olivia T ChengJianguo Cheng
Jul 21, 2009·Toxicon : Official Journal of the International Society on Toxinology·Alberto AmatoWiebe H C F Kooistra
Mar 9, 2005·Veterinary Pathology·P A SilvagniF M D Gulland
Aug 5, 2009·International Journal of Toxicology·Marion EhrichGunda Reddy
Jan 27, 2007·Analytical and Bioanalytical Chemistry·Pearse McCarronPhilipp Hess
Sep 28, 2006·Analytical and Bioanalytical Chemistry·Philipp HessMichael A Quilliam
Feb 23, 2000·Journal of Cardiovascular Pharmacology and Therapeutics·M S NijjarN S Dhalla
Oct 23, 2010·Journal of Agricultural and Food Chemistry·Yu HePhilippe Schmitt-Kopplin

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