The reliability of the measurement of plasma renin activity by radioimmunoassay

Clinica Chimica Acta; International Journal of Clinical Chemistry
J I Drayer, T J Benraad


A radioimmunoassay of angiotensin I has been applied to the measurement of plasma renin activity. Angiotensin I was generated in plasma samples by 3 h incubation at 37 degrees C and pH 5.6 after addition of EDTA and Dowex. The generated amount of angiotensin I was measured by radioimmunoassay in the eluate of the Dowex column. With this method a negligible amount of angiotensin I was measured after incubation at 4 degrees C (0.8 ng/ml per 3 h). Eluate of blank plasma had no measurable effect on the standard curve. The mean recovery of angiotensin I was 87%. The limit of detection of the assay was 0.5 ng/ml per 3 h. The results obtained using different antisera were equal. A marked variation was found in immunological properties of different standard preparations of angiotensin I tested. The mean value of angiotensin I generation per Goldblatt Unit (G.U.) renin was 3.9 with 10-4 ng/h. In normotensive control subjects, the plasma renin concentration, whileon unrestricted diet and after 3 h of ambulation, was on average 0.39 with 10-minus 4 G.U./ml, range 0.12 with 10-minus 4-0.91 with 10-minus 4. With the use of the same plasma extracts for radioimmunoassay and bioassay, a perfect correlation was found between the plasma renin ac...Continue Reading


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