PMID: 13575673Sep 1, 1958Paper

The removal of cartilage matrix, in vivo, by papain; identification of crystalline papain protease as the cause of the phenomenon

The Journal of Experimental Medicine
R T McCLUSKEY, L THOMAS

Abstract

The intravenous injection of crystalline papain into young rabbits results in depletion of cartilage matrix throughout the body, with loss of rigidity and collapse of the ears, provided the enzyme is inactivated by oxidation or sulfhydryl blocking agents prior to administration. Cysteine-activated crystalline papain, when injected intravenously, produces little or no change in cartilage. The changes which occur in cartilage following an injection of inactivated crystalline papain are indistinguishable from those produced by crude papain. Activation of crude papain by cysteine prior to injection results in loss of its capacity to produce in vivo changes in cartilage. The progressive changes which take place in cartilage in vivo also occur in vitro in isolated rabbit ears removed shortly after an injection of crude papain or inactivated crystalline papain. In vitro ear collapse occurs rapidly at 37 degrees C. and does not occur at 4 degrees C. Collapse is enhanced by exposing the cartilage to cysteine and prevented by exposure to iodoacetamide or p-chloromercuribenzoate. The direct action of crystalline papain on plates of normal cartilage, in vitro, results in the same gross and histological changes which were observed in vivo. ...Continue Reading

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Citations

Nov 1, 1970·Arthritis and Rheumatism·D S OwenE Toone
Jan 1, 1976·Mechanisms of Ageing and Development·F A Meyer, A Silberberg
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Sep 1, 1960·The Journal of Investigative Dermatology·R F MILLER, R B STOUGHTON

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