The repairing of full-thickness skin deficiency and its biological mechanism using decellularized human amniotic membrane as the wound dressing

Materials Science & Engineering. C, Materials for Biological Applications
Mengsheng SongYabin Zhu

Abstract

Human amniotic membrane (HAM) was a biocompatible scaffold with advantages of anti-inflammatory, low antigen, feasibility, tolerance and low cost. In our previous work, HAM was treated to be decellularized using surfactant, lipase and DNAase methods and the efficacy as an implantable biological mesh was verified after decellularization treatment. In this work, we used the previous protocol to decellularize the fresh HAM, and applied it to repair full-thickness skin defects with Sprague-Dawley rats as the test animals. The wound healing progress was followed in the duration of 8months, and the biological repairing mechanism was explored. From the wound area alteration, white blood cell (WBC) measurements and H&E staining, dHAM was detected to promote the wound healing, comparing with the traditional clinic treatment. Immunohistochemical analyses of the bio-factors involved in the wound healing, vascular endothelial growth factor (VEGF), alpha-smooth muscle actin (α-SMA) and transforming growth factor beta-1 (TGF-β1), exhibited that dHAM enhanced VEGF and α-SMA secretion but reduced TGF-β1 expression at early stage, which alleviated the wound inflammation, promoted the tissue regeneration and relieved the scar formation.

References

May 12, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Denys J LoeffelbeinMarco R Kesting
Oct 24, 2012·Experimental Dermatology·Jürgen HarderRegine Gläser
Dec 25, 2012·Fibrogenesis & Tissue Repair·Ludovic MicallefAlexis Desmoulière
Apr 9, 2013·Nanomedicine·Rúben F PereiraPaulo J Bartolo
Jul 3, 2013·ACS Applied Materials & Interfaces·Changfeng GongLin Luo
Jan 21, 2014·Current Topics in Developmental Biology·Ana Mafalda Baptista Tadeu, Valerie Horsley
Jan 25, 2014·Journal of Innate Immunity·Ilknur SenyürekBirgit Schittek
Feb 18, 2014·Cell and Tissue Banking·Hélène TauzinPatrice Muret
Sep 2, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Thomas J KoobMichelle Massee
Oct 11, 2014·Advances in Wound Care·Kelly E Johnson, Traci A Wilgus
Nov 22, 2014·Science·Bryan K SunPaul A Khavari
Mar 26, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Nagako YoshibaTakashi Okiji
Jun 6, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Peina ShiJun Wang
Jul 18, 2015·Clinical, Cosmetic and Investigational Dermatology·Thilo GambichlerLutz Schmitz
Jul 21, 2015·Burns : Journal of the International Society for Burn Injuries·Serag M ZidanMahmoud R Algamal
Jul 25, 2015·Journal of Translational Medicine·Neil H RiordanRandall W McKenna
Nov 30, 2015·BioMedicine·Selvaraj DhivyaElango Santhini
Aug 3, 2016·Plastic and Reconstructive Surgery. Global Open·Mihail ClimovThomas J Bollenbach
Jun 22, 2016·Journal of Developmental Biology·Richard W D GilbertAlicia M Viloria-Petit

❮ Previous
Next ❯

Citations

Dec 21, 2018·International Journal of Molecular Sciences·Andrea PorzionatoRaffaele De Caro
Apr 7, 2020·Journal of Biomaterials Science. Polymer Edition·Ilyas InciHuseyin Avci
Mar 20, 2019·Biomedical Engineering Online·Michael Meyer
Feb 2, 2021·Life Sciences·Ping ChenMehdi Aleahmad
Dec 31, 2020·Genes·Paz de la Torre, Ana I Flores
Sep 11, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Mathilde FenelonJean-Christophe Fricain
May 6, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Mathilde FenelonJean-Christophe Fricain
Jun 15, 2021·BioMed Research International·Hamideh MoravvejFahimeh Abdollahimajd
Jul 9, 2018·ACS Biomaterials Science & Engineering·Julien H Arrizabalaga, Matthias U Nollert
Oct 12, 2021·Frontiers in Bioengineering and Biotechnology·Melanie PichlsbergerIngrid Lang-Olip

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Related Papers

Materials Science & Engineering. C, Materials for Biological Applications
Peina ShiJun Wang
Acta Cirúrgica Brasileira
Ian Göedert Leite Duarte, Ivana Duval-Araujo
© 2022 Meta ULC. All rights reserved