The replication of a necrogenic cucumber mosaic virus satellite is temperature-sensitive in tomato

Archives of Virology
J L WhiteJ M Kaper

Abstract

Lethal necrosis development in tomato plants infected with cucumber mosaic virus (CMV) strain D containing the necrogenic satellite D-CARNA 5 and held at 32 degrees C is shown to be impaired. CARNA 5 accumulation in tomato at 32 degrees C is reduced about 100-fold compared to accumulation in plants held at 24 degrees C, while viral RNA accumulation is reduced about 5-fold. CMV-infected tomato held for 3 days at 24 degrees C prior to shift to 32 degrees C do not develop lethal necrosis. Longer incubations at 24 degrees C prior to shift to 32 degrees C allow necrosis to develop. CMV-infected plants held for up to 4 weeks at 32 degrees C required an additional 8-10 days at 24 degrees C to develop necrosis. Necrogenic CMV-infected plants held at 24 degrees C and analyzed 3 days p.i. contained detectable amounts of ss- and ds-CARNA 5; upon shift to 32 degrees C, such CARNA 5 declined to undetectable levels and lethal necrosis did not occur. There appear to be temperature-sensitive factors that are required for efficient satellite replication which are not required for efficient viral RNA replication. Whether these factor(s) are of host or satellite origin is uncertain.

References

Feb 1, 1989·Journal of Virological Methods·J L White, J M Kaper
Jan 1, 1985·Annual Review of Microbiology·R I Francki
Apr 22, 1977·Science·J M Kaper, H E Waterworth
Jun 1, 1979·Virology·D W Mossop, R I Francki

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Citations

May 18, 2006·Annual Review of Phytopathology·W Allen Miller, K Andrew White
Mar 15, 2006·Annual Review of Public Health·Thomas R Oliver

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