The replicative polymerases PolC and DnaE are required for theta replication of the Bacillus subtilis plasmid pBS72

Microbiology
Marina TitokL Jannière

Abstract

Plasmids are the tools of choice for studying bacterial functions involved in DNA maintenance. Here a genetic study on the replication of a novel, low-copy-number, Bacillus subtilis plasmid, pBS72, is reported. The results show that two plasmid elements, the initiator protein RepA and an iteron-containing origin, and at least nine host-encoded replication proteins, the primosomal proteins DnaB, DnaC, DnaD, DnaG and DnaI, the DNA polymerases DnaE and PolC, and the polymerase cofactors DnaN and DnaX, are required for pBS72 replication. On the contrary, the cellular initiators DnaA and PriA, the helicase PcrA and DNA polymerase I are dispensable. From this, it is inferred that pBS72 replication is of the theta type and is initiated by an original mechanism. Indirect evidence suggests that during this process the DnaC helicase might be delivered to the plasmid origin by the weakly active DnaD pathway stimulated by a predicted interaction between DnaC and a domain of RepA homologous to the major DnaC-binding domain of the cellular initiator DnaA. The plasmid pBS72 replication fork appears to require the same functions as the bacterial chromosome and the unrelated plasmid pAMbeta1. Most importantly, this replication machinery contain...Continue Reading

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Citations

May 17, 2007·PloS One·Laurent JannièreS Dusko Ehrlich
Nov 8, 2017·Critical Reviews in Biochemistry and Molecular Biology·Jeremy W SchroederJue D Wang
Aug 15, 2018·Antibiotics·Stefan IlicBarak Akabayov
Jan 24, 2019·Frontiers in Bioengineering and Biotechnology·Florian NadlerJohannes Kabisch

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