The reported germline repertoire of human immunoglobulin kappa chain genes is relatively complete and accurate.

Immunogenetics
A M CollinsW A Sewell

Abstract

We describe a bioinformatic analysis of germline and rearranged immunoglobulin kappa chain (IGK) gene sequences, performed in order to assess the completeness and reliability of the reported IGK repertoire. In contrast to the reported heavy-chain gene repertoire, which includes many dubious sequences, only five IGK variable gene (IGKV) alleles appear to have been reported in error. There was, however, insufficient evidence to justify removing these IGKV genes from the germline repertoire. Bioinformatic analysis of apparent mismatches between reported germline genes and 1,863 expressed IGK sequences suggested the existence of two unreported IGKV polymorphisms. Genomic screening of 12 individuals led to the confirmation of both of these polymorphisms, IGKV1-16*02 and IGKV2-30*02. We also show that in contrast to the heavy chain, the IGK repertoire is dominated by sequences that use just a handful of kappa variable (IGKV) and junction (IGKJ) gene pairs. There is also little modification of IGKV and IGKJ genes by the processes of exonuclease removal and N nucleotide addition. The expressed IGK repertoire therefore lacks diversity and the junction region is particularly constrained. Remarkably, the analysis of a dataset of 435 relat...Continue Reading

References

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Jun 6, 2006·The Journal of Allergy and Clinical Immunology·Isabell DahlkeAndrew M Collins
Jun 8, 2007·Journal of Immunological Methods·Katherine J L JacksonAndrew M Collins
Nov 28, 2007·Immunology and Cell Biology·Yan WangAndrew M Collins

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Citations

Dec 8, 2010·Immunome Research·Michal BarakRamit Mehr
Jul 27, 2011·Immunogenetics·Katherine Jean Louise JacksonAndrew M Collins
Sep 16, 2016·Frontiers in Immunology·Dongni HouYuanlin Song
Dec 21, 2017·Immunogenetics·Andrew M Collins, Katherine J L Jackson
Feb 2, 2020·Scientific Reports·Anne LjungarsMikael Mattsson
Mar 21, 2021·Communications Biology·M Frank ErasmusAndrew R M Bradbury

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