PMID: 8614264Jan 1, 1996Paper

The requirement for and mobilization of calcium during induction by sodium ascorbate and by hydrogen peroxide of cell death

Life Sciences
H SakagamiM Takeda

Abstract

The requirement for and mobilization of Ca2+ ions during induction of cell death by sodium ascorbate were compared with those during induction of cell death by hydrogen peroxide. When HL-60 cells were incubated with sodium ascorbate, a rapid increase in the intracellular concentration of Ca2+ ions and subsequent apoptotic cell death, characterized by cell shrinkage, nuclear fragmentation and cleavage of internucleosomal DNA to yield fragments that were multiples of 180-200 base pairs, were induced. However, these effects of sodium ascorbate were significantly reduced in Ca2+-depleted medium. By contrast, hydrogen peroxide induced similar apoptosis associated phenomena in the presence and in the absence of extracellular Ca2+ ions. The intracellular concentration of the reduced form of glutathione was not significantly affected and glutathione disulfide was undetectable during the early stages of apoptosis. These data suggest that sodium ascorbate and hydrogen peroxide initiate cell death by different mechanisms.

References

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Citations

Dec 31, 1998·Free Radical Biology & Medicine·H SakagamiM Maeda
Jan 1, 1997·Journal of Cancer Research and Clinical Oncology·M KurokawaM Adachi
May 23, 1998·Experimental Cell Research·F KebersA Noël
Aug 30, 2002·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Mauro MaccarroneMassimo Taccone-Gallucci
Aug 13, 2003·Free Radical Research·Hee Kyoung KangMyung Hee Chung
Jan 1, 1997·Free Radical Biology & Medicine·H SakagamiM Takeda

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis