The requirement for proteomics to unravel stem cell regulatory mechanisms

Journal of Cellular Physiology
Andrew J K Williamson, Anthony D Whetton

Abstract

Stem cells are defined by their ability to self-renew and to differentiate, the processes whereby these events are achieved is the subject of much investigation. These studies include cancer stem cell populations, where eradication of this specific population is the ultimate goal of treatment. Whilst cellular signalling events and transcription factor complex-mediated changes in gene expression have been analysed in some detail within stem cells, full systematic understanding of the events promoting self-renewal or the commitment process leading to formation of a specific cell type require a systems biology approach. This in turn demands a need for proteomic analysis of post-translational regulation of protein levels, protein interactions, protein post-translational modification (e.g. ubiquitination, methylation, acetylation, phosphorylation) to identify networks for stem cell regulation. Furthermore, the phenomenon of induced pluripotency via cellular reprogramming also can be understood optimally using combined molecular biology and proteomics approaches; here we describe current research employing proteomics and mass spectrometry to dissect stem cell regulatory mechanisms.

References

Jan 1, 1985·Annual Review of Biochemistry·T Hunter, J A Cooper
Apr 1, 1986·Analytical Biochemistry·L Andersson, J Porath
Mar 1, 1997·Electrophoresis·L Anderson, J Seilhamer
Jul 29, 1999·Analytical Chemistry·M C Posewitz, P Tempst
Sep 14, 2002·Science·Miguel Ramalho-SantosDouglas A Melton
Sep 14, 2002·Science·Natalia B IvanovaIhor R Lemischka
Sep 24, 2004·Molecular & Cellular Proteomics : MCP·Philip L RossDarryl J Pappin
Jun 1, 2005·Molecular & Cellular Proteomics : MCP·Richard D UnwinAnthony D Whetton
Jun 28, 2005·Molecular & Cellular Proteomics : MCP·William M OldNatalie G Ahn
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Mar 16, 2006·Analytical Chemistry·Hye Kyong Kweon, Kristina Håkansson
Jun 8, 2006·Proteomics·Hossein BaharvandGhasem Hosseini Salekdeh
Jul 25, 2006·Cell Cycle·Richard D Unwin, Anthony D Whetton
Aug 12, 2006·Expert Review of Proteomics·Dennis Van HoofJeroen Krijgsveld
Nov 10, 2006·Nature·Jianlong WangStuart H Orkin
Dec 13, 2006·Current Opinion in Chemical Biology·Benjamin A GarciaDonald F Hunt
Feb 13, 2007·Nature Methods·Bernd BodenmillerRuedi Aebersold
Feb 27, 2007·Cell·Tony Kouzarides
Mar 31, 2007·BMC Genomics·Kagnew Hailesellasse SeneMiguel A Andrade-Navarro
May 1, 2007·Regenerative Medicine·S RocheS Lehmann
Jul 27, 2007·Proteomics·Hye Won ParkChan-Wha Kim
Sep 25, 2007·Blood Reviews·Richard D Unwin, Anthony D Whetton
Nov 23, 2007·Journal of Cellular and Molecular Medicine·Nathan D PriceQiang Tian
Nov 30, 2007·Molecular & Cellular Proteomics : MCP·Andrew J K WilliamsonAnthony D Whetton
Dec 15, 2007·Nature Protocols·Kazutoshi TakahashiShinya Yamanaka
Dec 18, 2007·Molecular & Cellular Proteomics : MCP·Elaine SpooncerAnthony D Whetton
Dec 22, 2007·Nature·Ian ChambersAustin Smith
Dec 28, 2007·Experimental & Molecular Medicine·Moon-Young ChoiSung Won Kwon
Jan 17, 2008·Cells, Tissues, Organs·Jianlong Wang, Stuart H Orkin
Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·W E LowryK Plath
Mar 1, 2008·Journal of Proteome Research·Shabaz MohammedAlbert J R Heck

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Citations

Mar 20, 2013·BioMed Research International·Qinglei ZhanSaiming Ngai
Feb 27, 2014·Cellular Reprogramming·Suresh RamakrishnaKwang-Hyun Baek
Oct 30, 2016·Journal of Cellular Physiology·Jie LiJinlin Song
Jul 20, 2012·Expert Review of Proteomics·Onno KranenburgConnie R Jimenez

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