The requirement for Vif of SIVmac is cell-type dependent

The Journal of General Virology
J X Zou, P A Luciw

Abstract

The vif gene (viral infectivity factor) of the human and simian immunodeficiency viruses (HIV and SIV) is present in almost all members of the lentivirus group of retroviruses. This gene is highly conserved among different HIV and SIV isolates and is therefore presumed to play an important role in pathogenesis. To analyse the role of Vif in SIV, three SIVmac mutants have been constructed by introducing site-specific mutations or deletions into vif of the pathogenic molecular clone SIVmac239. The effect of Vif on viral replication in T cells was examined by transfecting equal amounts of either vif-positive or vif-negative viral DNA into SupT1, CEM-SS and H9 cells. Reverse transcriptase assay of supernatants from transfected cultures revealed that both SupT1 and CEM-SS cell lines supported replication of all three vif mutants to a level comparable to the parental vif-positive virus, whereas vif mutants did not replicate in H9 cells. Our results demonstrate that the requirement for Vif in SIVmac replication is cell-type dependent and that sequences near both the N and C termini are required for its function. Vif-defective SIVmac239, produced in transfected SupT1 and CEM-SS cells, failed to infect primary T lymphocytes, whereas bot...Continue Reading

Citations

Dec 1, 2001·Expert Opinion on Biological Therapy·S PandyaV Planelles
Jul 12, 2011·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Robert A Lever, Andrew M L Lever
Jun 26, 2012·Virus Research·Julien BatisseJean-Christophe Paillart
Oct 21, 2005·Journal of Molecular Biology·Simon HenrietRoland Marquet
Mar 12, 1999·Journal of Virology·S M WhiteV Planelles

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