PMID: 5343432Oct 1, 1969

The requirement of serine esterase function in complement-dependent erythrophagocytosis

The Journal of Experimental Medicine
D S PearlmanE L Becker

Abstract

The p-nitrophenyl ethyl phosphonate esters have been shown to inhibit complement-dependent erythrophagocytosis when exposed to guinea pig polymorphonuclear leukocytes prior to the initiation of phagocytosis. Inhibition of phagocytosis occurred in a manner characteristic of the well-defined capacity of phosphonate esters to inactivate serine esterases: inhibition was irreversible, dependent upon the temperature of reaction and pH of the reaction medium, and proportional to the concentration of inhibitor used and the duration of exposure between leukocytes and inhibitor. Phosphonate inhibition was further shown to be independent of any general cell damaging effects of the compounds used. The phagocytic enzyme inhibited by phosphonate esters apparently exists in or on leukocytes in an already activated state prior to the initiation of the phagocytic process. The inhibitory profile of the activated phagocytic esterase was found to be essentially identical to the profile of inhibition previously obtained for the activated chemotactic esterase of rabbit polymorphonuclear leukocytes, suggesting that the same enzyme may function in both chemotaxis and phagocytosis. Various substrates including acetate esters reported to protect the act...Continue Reading

References

Jun 1, 1967·The Journal of Experimental Medicine·P A Ward, E L Becker
Nov 1, 1968·The Journal of Experimental Medicine·P A Ward
Feb 1, 1965·The Journal of Experimental Medicine·P A Ward, C G Cochrane
Aug 1, 1965·The Journal of Experimental Medicine·P A WardH J MUELLER-EBERHARD

Citations

Jun 1, 1985·Inflammation·M F Tsan, M S Jiang
Apr 29, 1998·British Journal of Pharmacology·B E Khiav, F L Pearce
Jan 1, 1973·New Zealand Veterinary Journal·W E JonasS Broad
Sep 1, 1972·The Journal of Experimental Medicine·W P Arend, M Mannik
Sep 1, 1978·CRC Critical Reviews in Toxicology·M N Walters, J M Papadimitriou
Sep 1, 1979·Clinical Immunology and Immunopathology·A C IssekutzW D Biggar
Aug 1, 1970·Nature·A M Woodin, A A Wieneke
Feb 1, 1987·Biological Chemistry Hoppe-Seyler·N J Braun, H P Schnebli

Related Concepts

Metazoa
Chemotaxis
Hemolytic Complement
Enzyme Activation
Esterases
Esters
Cavia porcellus
Hydrogen-Ion Concentration
Leukocytes
Phagocytosis

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