Nov 6, 2018

The research progress of p53 tumour suppressor activity controlled by Numb in triple-negative breast cancer

BioRxiv : the Preprint Server for Biology
Xian JieYoude Cao

Abstract

Numb is known as a cell fate determinant. It has been shown to be a tumor suppressor, and there is a frequent loss of Numb expression in breast cancer. Numb prevents ubiquitination and degradation of p53.The aim of this study was to investigate the expression and migration of Numb, HDM2 and p53 in the membrane, cytoplasmic and nuclear fractions of MCF-10A and MDA-MB-231 cells. We extracted the cell fractions to detect changes of these three protein levels after re-expressing NUMB in MDA-MB-231 cells and knocking down NUMB in MCF-10A cells. Our results show that Numb can migrate from cytoplasm to nucleus of the MDA-MB-231 cells. Numb regulates p53 levels in nucleus of MCF-10A and MDA-MB-231 cells, and the Numb levels is positively correlated with p53 levels. We have a finding on HDM2 protein that it was remarkably reduced in the membrane fraction of NUMB knockdown cells, but its mRNA levels was significantly increased. We also examined the Numb expression in 125 patients with triple-negative breast cancer, 61(48.8%) displayed deficient or reduced expression of Numb.

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Mentioned in this Paper

TP53 gene
Study
Research
Tumor Suppressor Genes
MDM2 protein, human
MDM2 gene
Cell Fate
Gene Knockdown Techniques
Membrane
Membrane Fraction

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