The resistance and integrase genes of pACM1, a conjugative multiple-resistance plasmid, from Klebsiella oxytoca

Plasmid
K E PrestonR A Venezia

Abstract

pACM1 is an 85-kb conjugative plasmid from a clinical isolate of Klebsiella oxytoca that encodes resistance to beta-lactams (mediated by SHV-5 extended spectrum beta-lactamase), trimethoprim, sulfonamides, tetracycline, aminoglycosides, and mercuric chloride. The expression of the aminoglycoside resistance is difficult to detect, which could have clinical implications. A region of pACM1 containing five resistance genes and two putative integrons was characterized by restriction mapping and partial DNA sequencing. One integron appears to be class I (sull type); the second lacks a recognizable 3' conserved segment. Neither integron has the BamHI site predicted for the 5' conserved segment. Plasmids encoding SHV-5 from other bacterial strains appear to be closely related to pACM1 by restriction enzyme analysis, but have resistance/ integron regions that vary in size and content from that of pACM1. Integrase-mediated recombination might be responsible for genetic divergence in a widely distributed family of pACM1-like plasmids.

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Citations

Jul 18, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Stefano MorabitoAlessandra Carattoli
Sep 15, 2001·Antimicrobial Agents and Chemotherapy·E D PetersA C Fluit
Nov 26, 2003·Antimicrobial Agents and Chemotherapy·Hesna YigitFred C Tenover
Jun 25, 2003·Antimicrobial Agents and Chemotherapy·Karen E PrestonRichard A Venezia
Jan 28, 2005·Antimicrobial Agents and Chemotherapy·Louisa A JonesPeter A White
Jul 15, 2003·Clinical Microbiology Reviews·Sergei B Vakulenko, Shahriar Mobashery
May 24, 2006·Microbes and Infection·Vivi MiriagouSéamus Fanning
Sep 18, 1999·Plasmid·K E Preston, R A Venezia
Jun 13, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·U Garza-RamosJ Silva-Sanchez
Apr 23, 2002·Molecular Microbiology·Béatrice Hanau-BerçotEkkehard Collatz
Oct 6, 1999·Current Opinion in Microbiology·D A Rowe-Magnus, D Mazel
Dec 2, 2021·Clinical Microbiology Reviews·Jing YangZhiyong Zong

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