PMID: 9541455Apr 16, 1998Paper

The resistance of activated T-cells from SLE patients to apoptosis induced by human thymic stromal cells

Immunology Letters
V M BudagyanA A Yarylin

Abstract

In this paper we show the differential sensitivity of phytohemagglutinine (PHA) activated T-cells from healthy donors or patients with systemic lupus erythematosus (SLE) to apoptosis induced by human thymic stromal cell line of epithelial origin. T-cells from SLE patients were mainly resistant to the apoptotic action of the stromal cells, while normal T-lymphocytes readily died via apoptosis. Gel electrophoresis revealed a DNA fragmentation pattern characteristic of apoptosis after 18 h of coculture. The simultaneous measurement of [3H]-thymidine uptake showed that the proliferative response of T-cells from SLE patients was significantly decreased compared to their normal counterparts. Such difference may account for the distinct result of interactions between the stromal and lymphoid cells, leading to the subsequent survival of T-lymphocytes from SLE patients. Nevertheless pretreatment of normal activated T-lymphocytes with anti-Fas mAbs, which have the capacity to substantially inhibit signaling through this receptor resulted in abolition of this form of programmed cell death. Thus, the precise role of Fas receptor and its ligand in this in vitro test system needs further investigation.

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Citations

Aug 24, 1999·Current Opinion in Nephrology and Hypertension·J H BerdenW J Tax
Sep 2, 2015·Journal of Autoimmunity·Yogita Ghodke-Puranik, Timothy B Niewold
May 3, 2003·Annals of the New York Academy of Sciences·Philip L KongJoseph E Craft
Jun 10, 2010·Clinical and Experimental Immunology·W-U KimC-S Cho
Jul 28, 2011·Human Mutation·Jianming WuRobert P Kimberly
Jul 21, 2004·Rheumatic Diseases Clinics of North America·Sophie KoutouzovZahir Amoura
Dec 19, 2013·TheScientificWorldJournal·Bita MoudiZahra Zakeri
Feb 14, 2009·Lupus·A La Cava
Aug 13, 2009·Expert Opinion on Therapeutic Targets·Benjamin F Chong, Chandra Mohan
Oct 1, 2012·International Journal of Clinical Rheumatology·Ashley J Wilhelm, Amy S Major
Jan 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Farida BouzahzahJoseph Craft

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis