The resistance of electron-transport chain Fe-S clusters to oxidative damage during the reaction of peroxynitrite with mitochondrial complex II and rat-heart pericardium.

Nitric Oxide : Biology and Chemistry
Linda L PearceJim Peterson

Abstract

The effects of peroxynitrite and nitric oxide on the iron-sulfur clusters in complex II (succinate dehydrogenase) isolated from bovine heart have been studied primarily by EPR spectroscopy and no measurable damage to the constitutive 2Fe-2S, 3Fe-4S, or 4Fe-4S clusters was observed. The enzyme can be repeatedly oxidized with a slight excess of peroxynitrite and then quantitatively re-reduced with succinate. When added in large excess, peroxynitrite reacted with at least one tyrosine in each subunit of complex II to form 3-nitrotyrosines, but activity was barely compromised. Examination of rat-heart pericardium subjected to conditions leading to peroxynitrite production showed a small inhibition of complex II (16%) and a greater inhibition of aconitase (77%). In addition, experiments performed with excesses of sodium citrate and sodium succinate on rat-heart pericardium indicated that the "g approximately 2.01" EPR signal observed immediately following the beginning of conditions modeling oxidative/nitrosative stress, could be a consequence of both reversible oxidation of the constitutive 3Fe-4S cluster in complex II and degradation of the 4Fe-4S cluster in aconitase. However, the net signal envelope, which becomes apparent in le...Continue Reading

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Citations

Jul 11, 2012·Chemical Research in Toxicology·Kevin M NashFrederick A Villamena
Oct 6, 2010·Antioxidants & Redox Signaling·Anthony R Cyr, Frederick E Domann
Dec 18, 2012·International Journal of Molecular Sciences·Celia Harumi TenganRosely Oliveira Godinho
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Sep 29, 2020·Frontiers in Microbiology·Anna C ZemkeJames Peterson
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Feb 26, 2021·Metabolism: Clinical and Experimental·Christopher AugerMarc G Jeschke
Apr 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Xiaoting ZhouHongri Gong
Feb 6, 2018·Chemical Reviews·Gerardo Ferrer-SuetaRafael Radi

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