THE RESOLUTION OF CHLOROPHYLL a/b BINDING PROTEINS BY A PREPARATIVE METHOD BASED ON FLAT BED ISOELECTRIC FOCUSING

Photochemistry and Photobiology
P DaineseR Bassi

Abstract

We describe a new fractionation method for intrinsic membrane proteins based on flat bed isoelectric focusing (IEF) in granulated gel. The characteristics of the separation in the presence of the non-ionic detergent dodecylmaltoside are considered. The method has been applied to the fractionation of chlorophyll a/b binding proteins from chloroplast grana membranes. Several Light Harvesting Complexes II (LHC II) have been resolved showing differences in their polypeptide composition. Probing with monoclonal and polyclonal antibodies showed that polypeptides belonging to different [EF fractions with the same mobility in denaturing sodium dodecyl sulphate polyacrylamide gel electrophoresis, are immunologically distinct polypeptides. This is the first report of the presence in the thylakoid membrane of a number of LHCII polypeptides that may reflect the genetic complexity of the Cab genes. Moreover preparative amounts have been obtained of the minor chlorophyll a/b proteins CP 29, CP 26 and CP 24 that have been recently described. The analysis of a currently used LHCII preparation by the present method shows that this fraction is actually contaminated by two minor chlorophyll a/b proteins.

References

Mar 2, 1987·European Journal of Biochemistry·R Bassi, D Simpson
Nov 11, 1969·Biochimica Et Biophysica Acta·B J Radola
Jan 3, 1974·European Journal of Biochemistry·J P Thornber, H R Highkin

❮ Previous
Next ❯

Citations

May 15, 1996·European Journal of Biochemistry·E GiuffraR Bassi
Sep 1, 1993·Photosynthesis Research·P O ArvidssonH E Kerlund
Jun 7, 2006·Photosynthesis Research·Giorgio FortiGiuseppe Zucchelli
Feb 15, 1992·European Journal of Biochemistry·R Bassi, P Dainese
Mar 1, 1993·European Journal of Biochemistry·R BassiJ Marquardt
May 28, 2019·Planta·Anna M YeatesAlexander V Ruban
Jul 1, 1991·Journal of Basic and Clinical Physiology and Pharmacology·S YalovskyR Nechushtai
Jun 28, 2011·The Journal of Biological Chemistry·Alessandro AlboresiTomas Morosinotto
Dec 30, 2011·The Journal of Biological Chemistry·Giulia BonenteMatteo Ballottari
Apr 25, 2000·The Journal of Biological Chemistry·C JegerschöldR Bassi
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·R BassiD Sandonà
Apr 6, 2002·The Journal of Biological Chemistry·Paola DominiciRoberto Bassi
Jul 13, 2002·The Journal of Biological Chemistry·Tomas MorosinottoRoberto Bassi
Feb 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Morris O MakobongoMichael F Good
Mar 30, 1999·European Journal of Biochemistry·I AdamskaB Andersson
Oct 9, 1999·The Journal of Biological Chemistry·R CroceR Bassi
Nov 14, 2001·Journal of Photochemistry and Photobiology. B, Biology·G Jackowski, K Pielucha
Jun 12, 1999·Journal of Photochemistry and Photobiology. B, Biology·C De LucaR Bassi
Sep 28, 2002·Biochimica Et Biophysica Acta·Roberta CroceRoberto Bassi
Dec 30, 1994·Biochimica Et Biophysica Acta·S KimC F Yocum
Feb 8, 1994·Biochimica Et Biophysica Acta·S Jansson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.