PMID: 41573Nov 8, 1979

The respiratory chain of Paramecium tetraurelia in wild type and the mutant Cl1. II. Cyanide-insensitive respiration. Function and regulation

Biochimica Et Biophysica Acta
J DoussièreP V Vignais

Abstract

1. The cyanide-insensitive respiration in Paramecium tetraurelia was found to be located in mitochondria. 2. Sensitivity of the mitochondrial respiration to cyanide depended on growth conditions. Under standard conditions of growth, 15--20% of respiration was insensitive to 1 mM cyanide. Full resistance to 1 mM cyanide was observed by growing cells in the presence of erythromycin (100--400 microgram/ml) 0.2 mM cyanide. The mitochondrial respiration of the mutant Cl1 harvested during the exponential phase of growth was largely insensitive to cyanide (more than 80%). 3. Pyruvate was oxidized at the same rate by wild type mitochondria and mitochondria of the mutant Cl1. In contrast, succinate oxidation was 2--3 times faster in mitochondria of the mutant Cl1 than in wild type mitochondria. 4. The cyanide-insensitive respiration was inhibited by 1 mM salicylhydroxamic acid to nearly 100%. Other efficient respiratory inhibitors included amytal and heptylhydroxyquinoline. Antimycin was not inhibitory even at concentrations as high as 5 microgram/mg protein, a finding consistent with the lack of antimycin binding sites.

Citations

Oct 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A GhelliM Degli Esposti
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P Bennoun
Dec 1, 1988·Plant Physiology·B K Ishida, J M Palmer
Jan 25, 1982·FEBS Letters·P RiccioE Quagliariello
Aug 23, 1991·Biochimica Et Biophysica Acta·A L Moore, J N Siedow
Mar 11, 2020·International Journal of Molecular Sciences·Carlos FajardoFrancisco Javier Fernandez-Acero

Related Concepts

Isocyanides
Respiratory Chain
Hydrogen-Ion Concentration
Mitochondria
Oxygen Consumption
Paramecium

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