The Respiratory Microbiome in Chronic Hypersensitivity Pneumonitis Is Distinct from That of Idiopathic Pulmonary Fibrosis.

American Journal of Respiratory and Critical Care Medicine
Rachele InvernizziPhilip Molyneaux

Abstract

Rationale: Chronic hypersensitivity pneumonitis (CHP) is a condition that arises after repeated exposure and sensitization to inhaled antigens. The lung microbiome is increasingly implicated in respiratory disease, but, to date, no study has investigated the composition of microbial communities in the lower airways in CHP.Objectives: To characterize and compare the airway microbiome in subjects with CHP, subjects with idiopathic pulmonary fibrosis (IPF), and control subjects.Methods: We prospectively recruited individuals with a CHP diagnosis (n = 110), individuals with an IPF diagnosis (n = 45), and control subjects (n = 28). Subjects underwent BAL and bacterial DNA was isolated, quantified by quantitative PCR and the 16S ribosomal RNA gene was sequenced to characterize the bacterial communities in the lower airways.Measurements and Main Results: Distinct differences in the microbial profiles were evident in the lower airways of subjects with CHP and IPF. At the phylum level, the prevailing microbiota of both subjects with IPF and subjects with CHP included Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria. However, in IPF, Firmicutes dominated, whereas the percentage of reads assigned to Proteobacteria in the same...Continue Reading

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Citations

Sep 3, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jay H LipinskiDavid N O'Dwyer
Jan 16, 2021·Journal of Inflammation Research·Jacob E Michalski, David A Schwartz
Aug 14, 2020·American Journal of Respiratory and Critical Care Medicine·David N O'Dwyer, Stavros Garantziotis
May 1, 2021·Biomedicines·Paschalis NtoliosPaschalis Steiropoulos
Mar 4, 2021·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christoph TabelingMartin Witzenrath
Jun 8, 2021·Frontiers in Immunology·Thomas Planté-BordeneuveAntoine Froidure

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Datasets Mentioned

BETA
PRJNA609242

Methods Mentioned

BETA
PCR
PCA

Software Mentioned

R
Quantitative Insights into Microbial Ecology
R Foundation for Statistical Computing
PERMANOVA

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