The Response of Enterococcus faecalis V583 to Chloramphenicol Treatment.

International Journal of Microbiology
Agot AakraIngolf F Nes

Abstract

Many Enterococcus faecalis strains display tolerance or resistance to many antibiotics, but genes that contribute to the resistance cannot be specified. The multiresistant E. faecalis V583, for which the complete genome sequence is available, survives and grows in media containing relatively high levels of chloramphenicol. No specific genes coding for chloramphenicol resistance has been recognized in V583. We used microarrays to identify genes and mechanisms behind the tolerance to chloramphenicol in V583, by comparison of cells treated with subinhibitory concentrations of chloramphenicol and untreated V583 cells. During a time course experiment, more than 600 genes were significantly differentially transcribed. Since chloramphenicol affects protein synthesis in bacteria, many genes involved in protein synthesis, for example, genes for ribosomal proteins, were induced. Genes involved in amino acid biosynthesis, for example, genes for tRNA synthetases and energy metabolism were downregulated, mainly. Among the upregulated genes were EF1732 and EF1733, which code for potential chloramphenicol transporters. Efflux of drug out of the cells may be one mechanism used by V583 to overcome the effect of chloramphenicol.

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Citations

Jun 12, 2013·PloS One·Jacqueline AbranchesJosé A Lemos
Jul 7, 2016·Antimicrobial Agents and Chemotherapy·Lea M HürlimannMarkus A Seeger
Dec 7, 2011·Antimicrobial Agents and Chemotherapy·Matilde FernándezEstrella Duque

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Datasets Mentioned

BETA
GM17
EF0633

Methods Mentioned

BETA
acetylation
reverse transcription PCR
transcription

Software Mentioned

GenePix Pro
R
LIMMA
GenePix

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