PMID: 8598053Mar 1, 1996Paper

The response regulator-like protein Pos9/Skn7 of Saccharomyces cerevisiae is involved in oxidative stress resistance

Current Genetics
B KremsK D Entian

Abstract

We have isolated mutants of Saccharomyces cerevisiae with an increased sensitivity to oxidative stress. All pos9 mutants (pos for peroxide sensitivity) were hypersensitive to methylviologene, hyperbaric oxygen or hydrogen peroxide, but grew similarly to the wild-type under all other conditions tested. Isolation and sequencing of the respective POS9 gene revealed that it was identical to SKN7. The predicted Skn7/Pos9 protein possesses a domain with high homology to prokaryotic response regulators. These regulatory proteins are part of a simple signalling cascade termed a "two-component system", where a phosphorylation signal of a histidine kinase is transferred to a conserved aspartate residue of the response regulator. To test the functional role of the respective aspartate residue of Skn7/Pos9 protein in oxidative stress, we mutagenized this residue in vitro to alanine, arginine and glutamate. Only the glutamate allele (D427 to E) was able to rescue the hydrogen peroxide-sensitivity of pos9 mutants. By fusion experiments with the Gal4 DNA-binding domain we identified the isolated response regulator-like domain as a novel eukaryotic domain sufficient for gene activation. Whereas this hybrid protein activated transcription of a ...Continue Reading

References

Sep 8, 1978·Science·I Fridovich
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1991·Yeast·P M Sharp, E Cowe
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E B GrallaJ S Valentine
Feb 1, 1989·Molecular and Cellular Biology·M S Szczypka, D J Thiele
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·O Bermingham-McDonoghJ S Valentine
Nov 11, 1971·European Journal of Biochemistry·D E HallP Reichard
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M KarinS Fogel
Jun 4, 1993·Cell·J S Parkinson
Oct 22, 1993·Science·I M Ota, A Varshavsky

❮ Previous
Next ❯

Citations

Apr 20, 2004·FEMS Yeast Research·Aurora Zuzuarregui, Marcel-lí del Olmo
May 29, 2002·Biochimica Et Biophysica Acta·Ulla H DormerDerek J Jamieson
Dec 1, 2007·The Journal of Biological Chemistry·Sandhya ShankarnarayanJan S Fassler
Mar 24, 2009·Eukaryotic Cell·Xin-Jian HeJan S Fassler
Dec 7, 2010·Eukaryotic Cell·Jan S Fassler, Ann H West
Oct 30, 2007·Eukaryotic Cell·Eve LenssenMartine A Collart
Jul 26, 2005·Infection and Immunity·F L WormleyG M Cox
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Stefan Hohmann
Mar 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Katherine A BorkovichRobert Pratt
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·David E Levin
Apr 8, 2010·Biochemistry. Biokhimii︠a︡·V I Lushchak
Sep 1, 2009·Microbial Cell Factories·M Isabel González-SisoM Esperanza Cerdán
Oct 3, 2012·Genetics·Haruo Saito, Francesc Posas
Mar 11, 1999·Current Opinion in Genetics & Development·W M Toone, N Jones
Dec 9, 2000·Molecular Biology and Evolution·K K KoretkeJ R Brown
Mar 24, 2016·Bioscience, Biotechnology, and Biochemistry·Daisuke HagiwaraKatsuya Gomi
Jan 6, 2001·Redox Report : Communications in Free Radical Research·P Moradas-Ferreira, V Costa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.