The Responses of Tissues from the Brain, Heart, Kidney, and Liver to Resuscitation following Prolonged Cardiac Arrest by Examining Mitochondrial Respiration in Rats

Oxidative Medicine and Cellular Longevity
Junhwan KimLance B Becker

Abstract

Cardiac arrest induces whole-body ischemia, which causes damage to multiple organs. Understanding how each organ responds to ischemia/reperfusion is important to develop better resuscitation strategies. Because direct measurement of organ function is not practicable in most animal models, we attempt to use mitochondrial respiration to test efficacy of resuscitation on the brain, heart, kidney, and liver following prolonged cardiac arrest. Male Sprague-Dawley rats are subjected to asphyxia-induced cardiac arrest for 30 min or 45 min, or 30 min cardiac arrest followed by 60 min cardiopulmonary bypass resuscitation. Mitochondria are isolated from brain, heart, kidney, and liver tissues and examined for respiration activity. Following cardiac arrest, a time-dependent decrease in state-3 respiration is observed in mitochondria from all four tissues. Following 60 min resuscitation, the respiration activity of brain mitochondria varies greatly in different animals. The activity after resuscitation remains the same in heart mitochondria and significantly increases in kidney and liver mitochondria. The result shows that inhibition of state-3 respiration is a good marker to evaluate the efficacy of resuscitation for each organ. The resul...Continue Reading

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Citations

Jul 7, 2017·Circulation Journal : Official Journal of the Japanese Circulation Society·UNKNOWN SOS-KANTO 2012 Study Group
Nov 24, 2016·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Junhwan KimLance B Becker
Jul 1, 2018·Journal of the American Heart Association·Koichiro ShinozakiJoshua W Lampe
Sep 4, 2020·Cell Stress & Chaperones·Yifan Chen, Tianzheng Yu
Mar 30, 2021·Journal of the American Heart Association·Yu OkumaKoichiro Shinozaki
May 22, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xianfei JiWanchun Tang

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