The restricted potential for recovery of growth plate chondrogenesis and longitudinal bone growth following exposure to pro-inflammatory cytokines

The Journal of Endocrinology
V E MacRaeS F Ahmed

Abstract

Childhood chronic inflammatory disease can be associated with transient and permanent growth retardation. This study examined the potential for spontaneous growth recovery following pro-inflammatory cytokine exposure. Murine ATDC5 chondrogenic cells and postnatal metatarsals were exposed to interleukin (IL)-1beta, IL-6 and tumour necrosis factor-alpha (TNFalpha), and their growth and proliferative capacity were determined following recovery. TNFalpha and IL-1beta reduced chondrocyte proliferation and aggrecan and collagen types II and X expression at minimum concentrations of 10 ng/ml and 0.1 ng/ml respectively. TNFalpha but not IL-1beta exposure led to increased caspase-3 activity and altered cellular morphology, consistent with reduced viability. Cytokine exposure particularly inhibited proteoglycan synthesis. This effect was dose and duration dependent. Compared with the control, IL-1beta and TNFalpha led to a 71% and 45% reduction in metatarsal growth after 8 days of exposure respectively (P < 0.05). An additive effect of IL-1beta combined with TNFalpha was observed (110% decrease; P < 0.05). Metatarsals exposed to IL-1beta or TNFalpha individually for a 2-day period, and allowed to recover spontaneously in the absence of c...Continue Reading

Citations

Aug 14, 2012·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Colin Farquharson, S Faisal Ahmed
Oct 12, 2012·Endocrine·Tiziana MontalciniArturo Pujia
Mar 17, 2010·Pediatric Rheumatology Online Journal·Takako MiyamaeShumpei Yokokta
Dec 8, 2011·Journal of Pediatric Endocrinology & Metabolism : JPEM·Tamara S HannonScott C Denne
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Aug 24, 2013·Arthritis Research & Therapy·Ellie B M LandmanMarcel Karperien
Mar 22, 2014·International Journal of Endocrinology·Cosimo GianniniFrancesco Chiarelli
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