The Ret receptor regulates sensory neuron dendrite growth and integrin mediated adhesion

ELife
Peter SobaYuh Nung Jan

Abstract

Neurons develop highly stereotyped receptive fields by coordinated growth of their dendrites. Although cell surface cues play a major role in this process, few dendrite specific signals have been identified to date. We conducted an in vivo RNAi screen in Drosophila class IV dendritic arborization (C4da) neurons and identified the conserved Ret receptor, known to play a role in axon guidance, as an important regulator of dendrite development. The loss of Ret results in severe dendrite defects due to loss of extracellular matrix adhesion, thus impairing growth within a 2D plane. We provide evidence that Ret interacts with integrins to regulate dendrite adhesion via rac1. In addition, Ret is required for dendrite stability and normal F-actin distribution suggesting it has an essential role in dendrite maintenance. We propose novel functions for Ret as a regulator in dendrite patterning and adhesion distinct from its role in axon guidance.

References

Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R S PaulesJ J Eppig
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T C LairmoreH Donis-Keller
Dec 18, 2001·The Journal of Comparative Neurology·W B GrueberJ W Truman
Mar 12, 2002·The Journal of Biological Chemistry·Toshifumi FukudaMasahide Takahashi
Mar 29, 2002·Nature·Satoko Hakeda-SuzukiBarry J Dickson
Mar 29, 2002·Nature·Julian NgLiqun Luo
Jun 21, 2005·Genetics·Renee D ReadRoss L Cagan
Aug 17, 2006·Brain, Behavior and Evolution·Matti S AiraksinenTuomas Hätinen
Apr 11, 2007·Trends in Cell Biology·Joaquim Egea, Rüdiger Klein
May 8, 2007·Neuron·Michael E HughesDietmar Schmucker
May 8, 2007·Cell·Benjamin J MatthewsWesley B Grueber
Oct 16, 2007·Annals of Medicine·Pia Runeberg-Roos, Mart Saarma
Oct 30, 2007·Journal of Medical Genetics·J AmielUNKNOWN Hirschsprung Disease Consortium
Dec 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sebastian KowskyJörg B Schulz
Jan 25, 2008·Nature·Peter G FuerstRobert W Burgess
Jun 10, 2008·Nature Methods·Julia RiedlRoland Wedlich-Soldner
Sep 27, 2008·Developmental Biology·Svetla DimitrovaGaia Tavosanis
Feb 24, 2010·The Journal of Cell Biology·Tom T ChenM Luisa Iruela-Arispe
Apr 21, 2010·Nature Reviews. Neuroscience·Yuh-Nung Jan, Lily Yeh Jan
Jun 25, 2010·Cold Spring Harbor Perspectives in Biology·Wesley B Grueber, Alvaro Sagasti
Aug 13, 2010·The Journal of Clinical Endocrinology and Metabolism·Jessica G CockburnLois M Mulligan
Oct 30, 2010·Cell·S Lawrence Zipursky, Joshua R Sanes
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Chun HanYuh-Nung Jan
Jun 15, 2011·Annual Review of Cell and Developmental Biology·Johanna Ivaska, Jyrki Heino
Aug 30, 2011·Current Biology : CB·Benjamin J Matthews, Wesley B Grueber

❮ Previous
Next ❯

Citations

May 3, 2016·Current Opinion in Neurobiology·Caitlin A ShortTimothy M Gomez
Aug 25, 2016·Frontiers in Cellular Neuroscience·Fernanda Ledda, Gustavo Paratcha
Sep 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Amy R PoeChun Han
Nov 11, 2017·Journal of Cell Science·Brian V JenkinsJill Wildonger
Mar 28, 2020·Biotechnology Progress·Hannah ReeseStefano Menegatti
Jan 23, 2019·Frontiers in Physiology·Lois M Mulligan
Jun 24, 2016·Expert Review of Proteomics·Lois M Mulligan
Oct 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Adam W AveryThomas S Hays
Nov 27, 2018·F1000Research·Shalini Menon, Stephanie Gupton
May 20, 2020·Cellular and Molecular Bioengineering·Mohan Yasodharababu, Arun K Nair
Mar 24, 2020·Journal of Proteome Research·Katrin BrenigGereon Poschmann
Apr 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Grace Ji-Eun ShinWesley B Grueber
Jun 18, 2021·Frontiers in Cellular Neuroscience·Chang YinJay Z Parrish
Oct 3, 2021·G3 : Genes - Genomes - Genetics·Cristina M OstaléJose F de Celis

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPase
co-immunoprecipitation
GTPases
transgenic
affinity purification
confocal microscopy
transfection

Software Mentioned

BitPlane
Imaris Tracer
Imaris Filament Tracer
LifeAct
Origin Pro
Imaris Coloc
AutoQuant
Fiji
ImageJ

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Axon Guidance

Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.