The retinitis pigmentosa protein RP2 links pericentriolar vesicle transport between the Golgi and the primary cilium

Human Molecular Genetics
R Jane EvansMichael E Cheetham

Abstract

Photoreceptors are complex ciliated sensory neurons. The basal body and periciliary ridge of photoreceptors function in association with the Golgi complex to regulate the export of proteins from the inner segment to the outer segment sensory axoneme. Here, we show that the retinitis pigmentosa protein RP2, which is a GTPase activating protein (GAP) for Arl3, localizes to the ciliary apparatus, namely the basal body and the associated centriole at the base of the photoreceptor cilium. Targeting to the ciliary base was dependent on N-terminal myristoylation. RP2 also localized to the Golgi and periciliary ridge of photoreceptors, which suggested a role for RP2 in regulating vesicle traffic and docking. To explore this hypothesis, we investigated the effect of RP2 depletion and the expression of a constitutively active form of Arl3 (Q71L) on pericentriolar vesicle transport. Kif3a, a component of intraflagellar transport (IFT), is important in cilia maintenance and transport of proteins through the connecting cilium in photoreceptors. Similar to Kif3a and Arl3 depletion, loss of RP2 led to fragmentation of the Golgi network. Depletion of RP2 and dysregulation of Arl3 resulted in dispersal of vesicles cycling cargo from the Golgi c...Continue Reading

References

Jul 26, 1996·Cell·G TianN J Cowan
Aug 11, 1998·Nature Genetics·U SchwahnW Berger
Feb 16, 2002·The Journal of Biological Chemistry·Francesca BartoliniNicholas J Cowan
Mar 28, 2002·The Journal of Cell Biology·Gregory J PazourJoseph C Besharse
Nov 5, 2002·Nature Reviews. Molecular Cell Biology·Joel L Rosenbaum, George B Witman
Dec 24, 2002·Trends in Cell Biology·Gregory J Pazour, Joel L Rosenbaum
Jun 2, 2005·The Journal of Cell Biology·Lynne M Quarmby, Jeremy D K Parker
Jul 7, 2005·The Journal of Cell Biology·Gregory J PazourGeorge B Witman
Jul 7, 2005·American Journal of Physiology. Renal Physiology·Manish M TiwariPhilip R Mayeux
Nov 9, 2005·American Journal of Physiology. Renal Physiology·James R Davenport, Bradley K Yoder
Dec 13, 2005·Nature Reviews. Genetics·Friedhelm Hildebrandt, Edgar Otto
Mar 10, 2006·Molecular Biology of the Cell·Chengjing ZhouRichard A Kahn
Mar 28, 2006·The American Journal of Pathology·Jeffrey J SchrickDennis S Rice
Oct 27, 2006·Investigative Ophthalmology & Visual Science·David JimenoDavid S Williams
Nov 23, 2006·Current Biology : CB·Tobias StauberIsabelle Vernos
Jan 24, 2007·Traffic·Søren T ChristensenPeter Satir
Sep 27, 2007·Ophthalmic Genetics·N A AdamsHassanain S Toma
Oct 24, 2007·Sub-cellular Biochemistry·Ronald Roepman, Uwe Wolfrum
Oct 24, 2007·Nature Reviews. Molecular Cell Biology·Manfred FliegaufHeymut Omran
Oct 30, 2007·Neurobiology of Disease·Saya NakagomiStuart A Lipton
Mar 21, 2008·Nature Reviews. Molecular Cell Biology·Maria Antonietta De Matteis, Alberto Luini
Apr 1, 2008·Nature Structural & Molecular Biology·Stefan VeltelAlfred Wittinghofer
Oct 24, 2009·Journal of the American Society of Nephrology : JASN·Binli TaoLisa M Guay-Woodford

❮ Previous
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Citations

May 13, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Mathieu Boissan, Marie-Lise Lacombe
Feb 22, 2011·Protein & Cell·Yujie Li, Jinghua Hu
May 19, 2011·Nature Reviews. Molecular Cell Biology·Julie G Donaldson, Catherine L Jackson
Nov 22, 2013·The Journal of Biological Chemistry·Jane AndrePaul G McKean
Mar 30, 2013·Zebrafish·Rakesh Kotapati RaghupathyXinhua Shu
Feb 4, 2011·Molecular Biology of the Cell·Cary K LaiSuzie J Scales
Jun 22, 2012·Molecular Biology of the Cell·Qian HeErhard Bieberich
Nov 11, 2011·Human Molecular Genetics·Nele SchwarzMichael E Cheetham
Jul 31, 2012·Human Molecular Genetics·Alejandro Estrada-CuzcanoDorus A Mans
Sep 12, 2012·Human Molecular Genetics·Emma RichetJacqueline van der Spuy
Aug 15, 2013·Human Molecular Genetics·Yan ZhangVal C Sheffield
Nov 5, 2011·Genes & Development·R Tyler HillmanMatthew P Scott
Nov 17, 2011·Genes & Development·Kevin J WrightPeter K Jackson
Feb 3, 2011·Journal of Cell Science·Toby W HurdBen L Margolis
Dec 18, 2013·Human Molecular Genetics·Laurence DuplombChristel Thauvin-Robinet
Jun 13, 2012·The Journal of Cell Biology·Francesc R Garcia-Gonzalo, Jeremy F Reiter
Aug 20, 2015·American Journal of Medical Genetics. Part a·Nuria Garcia SegarraLuisa Bonafé
Apr 15, 2011·Biology of the Cell·Yi Shan LimBor Luen Tang
Mar 6, 2012·Journal of Cellular Biochemistry·Yujie LiJinghua Hu
Apr 10, 2013·Traffic·Elsa SeixasDuarte C Barral
Mar 18, 2016·Cellular and Molecular Neurobiology·Juan Wang, Maureen M Barr
Jul 26, 2014·Clinical & Experimental Ophthalmology·Ivan ProkudinHakon Hakonarson
Mar 5, 2016·Human Molecular Genetics·Zachary C WrightVisvanathan Ramamurthy
Jun 15, 2010·Current Opinion in Microbiology·Eva GluenzPaul G McKean
Apr 12, 2016·Nature Communications·Eyad Kalawy FansaShehab Ismail
Apr 9, 2013·Progress in Retinal and Eye Research·Jillian N PearringVadim Y Arshavsky
Aug 28, 2012·Vision Research·Ozge Yildiz, Hemant Khanna
Jun 5, 2015·Molecular Biology of the Cell·Julie E MaguireMaureen M Barr
Oct 29, 2013·Organogenesis·Gabrielle WhewayColin A Johnson
Apr 1, 2014·American Journal of Human Genetics·Said El ShamiehIsabelle Audo
May 6, 2014·American Journal of Human Genetics·Panagiotis I SergouniotisVincent Plagnol
Aug 14, 2012·Vision Research·Nele SchwarzMichael E Cheetham
Mar 24, 2015·PloS One·Juan Hidalgo-de-QuintanaJacqueline van der Spuy
Nov 3, 2016·Small GTPases·Eyad K Fansa, Alfred Wittinghofer

❮ Previous
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