The retrohoming of linear group II intron RNAs in Drosophila melanogaster occurs by both DNA ligase 4-dependent and -independent mechanisms.

PLoS Genetics
Travis B White, Alan M Lambowitz

Abstract

Mobile group II introns are bacterial retrotransposons that are thought to have invaded early eukaryotes and evolved into introns and retroelements in higher organisms. In bacteria, group II introns typically retrohome via full reverse splicing of an excised intron lariat RNA into a DNA site, where it is reverse transcribed by the intron-encoded protein. Recently, we showed that linear group II intron RNAs, which can result from hydrolytic splicing or debranching of lariat RNAs, can retrohome in eukaryotes by performing only the first step of reverse splicing, ligating their 3' end to the downstream DNA exon. Reverse transcription then yields an intron cDNA, whose free end is linked to the upstream DNA exon by an error-prone process that yields junctions similar to those formed by non-homologous end joining (NHEJ). Here, by using Drosophila melanogaster NHEJ mutants, we show that linear intron RNA retrohoming occurs by major Lig4-dependent and minor Lig4-independent mechanisms, which appear to be related to classical and alternate NHEJ, respectively. The DNA repair polymerase θ plays a crucial role in both pathways. Surprisingly, however, mutations in Ku70, which functions in capping chromosome ends during NHEJ, have only moder...Continue Reading

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Citations

Oct 31, 2015·Cellular and Molecular Life Sciences : CMLS·Kelly Beagan, Mitch McVey
Mar 17, 2015·Nature Structural & Molecular Biology·Karl E ZahnSylvie Doublié
Oct 3, 2014·PLoS Genetics·Matthew J YousefzadehRichard D Wood
Jun 25, 2015·Microbiology Spectrum·Huatao GuoJeff F Miller
Jun 25, 2015·Microbiology Spectrum·Steven Zimmerly, Li Wu
Jun 25, 2015·Microbiology Spectrum·Alan M Lambowitz, Marlene Belfort

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Methods Mentioned

BETA
PCR
PCRs
transgenic
electrophoresis

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