The return of phosphorylated and nonphosphorylated epitopes of neurofilament proteins to the regenerating optic nerve of Xenopus laevis

The Journal of Comparative Neurology
Y Zhao, B G Szaro

Abstract

Neurofilament proteins of mammalian axotomized peripheral axons, which regenerate effectively, resemble those of embryonic axons. However, injured centrally projecting mammalian axons, which fail to regenerate, have very different neurofilament compositions than during development. If changes in neurofilament composition after injury reflect the ability of axotomized neurons to regenerate effectively, then the neurofilaments of centrally projecting axons that can regenerate should more closely resemble those of developing axons. In this study, the neurofilament compositions of injured optic axons of the frog, Xenopus laevis, were examined, since these axons can regenerate a fully functional projection. Antibodies to phosphorylated and nonphosphorylated forms of neurofilament proteins that had been used previously to study the neurofilament composition of newly developing X. laevis optic axons were used in immunocytochemical studies to examine the return of neurofilaments to the optic nerve after an intraorbital nerve crush. Intraocularly injected wheat germ agglutinin conjugated to horseradish peroxidase was used to label the regenerating axons independently of their neurofilaments. Neurofilament immunoreactivities disappeared ...Continue Reading

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Citations

Feb 24, 2001·The European Journal of Neuroscience·C LodovichiL Maffei
Apr 1, 2018·The Journal of Comparative Neurology·Gabriela Edwards-FaretJuan Larraín
Nov 10, 2010·The European Journal of Neuroscience·Kurt M GibbsBen G Szaro
Sep 6, 2019·International Journal of Molecular Sciences·Marie ClaesLieve Moons
May 2, 2003·The Journal of Comparative Neurology·Christine GervasiBen G Szaro
Mar 21, 2020·Development·Lauren S PhippsEnrique Amaya
Aug 30, 2008·Development·Yuanyuan LiuBen G Szaro
Feb 1, 2000·Differentiation; Research in Biological Diversity·D LeakeN Schechter
Mar 31, 2021·Cold Spring Harbor Protocols·Paula G SlaterJuan Larraín
Apr 13, 2007·Experimental Cell Research·Amar ThyagarajanBen G Szaro

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