The reversal of hyperglycaemia in diabetic mice using PLGA scaffolds seeded with islet-like cells derived from human embryonic stem cells

Biomaterials
Gen-hong MaoYan-xia Wang

Abstract

Islet-like cells derived from embryonic stem (ES) cells may be a promising therapeutic option for future diabetes treatment. Here, we demonstrated a five-stage protocol with adding exendin-4 instead of nicotinamide finally could generate islet-like cells from human embryonic stem (ES) cells. Immunofluorescence analysis revealed a high percentage of c-peptide positive cells in the derivation. However, in addition to insulin/c-peptide, most cells also coexpressed PDX-1 (pancreas duodenum homeobox-1), glucagon, somatostatin or pancreatic polypeptide. Insulin and other pancreatic beta-cell-specific genes were all present in the differentiated cells. Insulin secretion could be detected and increased significantly by adding KCL in high glucose concentration in vitro. Furthermore, subcutaneous transplantation of scaffolds seeded with the islet-like cells or cell transplantation under kidney capsules for further differentiation in vivo could improve 6h fasted blood glucose levels and diabetic phenotypes in streptozotocin-induced diabetic SCID mice. More interestingly, blood vessels of host origin, characterized by mouse CD31 immunostaining, invaded the cell-scaffold complexes. This work reveals a five-stage protocol with adding exendin...Continue Reading

Citations

Feb 15, 2012·Annals of Surgery·Giuseppe OrlandoShay Soker
Jun 8, 2012·Cold Spring Harbor Perspectives in Medicine·Matthias Hebrok
Jan 17, 2013·World Journal of Gastroenterology : WJG·Marco CarboneJames Neuberger
Dec 1, 2012·Stem Cells Translational Medicine·Philippe A LysySusan Bonner-Weir
Mar 29, 2014·Expert Opinion on Drug Delivery·Tarun Garg, Amit K Goyal
Mar 20, 2012·Journal of Bioscience and Bioengineering·Su-Jung KimDong-Il Kim
Dec 15, 2010·Biochemical and Biophysical Research Communications·Yun ZhangMin Deng
Nov 3, 2010·Annals of the New York Academy of Sciences·Jeremy A Lavine, Alan D Attie
Nov 11, 2010·Transplant International : Official Journal of the European Society for Organ Transplantation·Giuseppe OrlandoShay Soker
Jun 10, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·K A HlavatyL D Shea
Mar 13, 2014·Annals of the New York Academy of Sciences·Jacqueline V Schiesser, James M Wells
Sep 22, 2009·Stem Cell Research·Dennis Van HoofMichael S German
Feb 6, 2015·Pediatric Diabetes·Nick Giannoukakis, Massimo Trucco
Mar 5, 2014·Organogenesis·Nobuhiko Kojima
Jul 20, 2012·International Journal of Cardiology·Meijuan GaoTianpei Hong
Nov 18, 2015·Journal of Diabetes Research·Seyed Jafar HashemianEnsieh Nasli-Esfahani
Feb 4, 2010·Advanced Drug Delivery Reviews·Will W MinuthAnne Glashauser
Aug 20, 2010·Cell Transplantation·Jamal DaoudMaryam Tabrizian
Aug 26, 2018·Biotechnology and Bioengineering·Marcos Perez-BasterrecheaAlvaro J Obaya
Aug 12, 2017·Frontiers in Bioengineering and Biotechnology·Silvia CaddeoSusanna Sartori
Jun 6, 2018·Frontiers in Cell and Developmental Biology·Piyaporn RattananinsruangWilairat Leeanansaksiri
Apr 25, 2020·Nanomaterials·Rosita PrimaveraAvnesh S Thakor
Jul 6, 2019·Current Diabetes Reports·Juewan KimP Toby Coates

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