The reversibility of nitrobenzene-induced testicular toxicity: continuous monitoring of sperm output from vasocystotomized rats

Toxicology
A A LevinB E Butterworth

Abstract

Exposure of rats to nitrobenzene produces a degeneration of the seminiferous epithelium of the testes. Sperm production was continuously monitored in rats surgically prepared by anastomosing the vas deferentia with the urinary bladder to evaluate the reversibility of nitrobenzene toxicity. Rates of sperm production were monitored by collecting urine and counting sperm microscopically with a hemocytometer. Six weeks after surgery, rats were dosed p.o. with a single dose of 300 mg/kg of nitrobenzene in corn oil. Sperm were not detected in the urine of treated rats between 32 and 48 days after treatment. Despite the fact that degenerative changes in the seminiferous tubules were observed histologically as early as 3 days after dosing, there was a 32-day lag period between treatment and cessation of sperm output in treated rats. Histological examination showed that pachytene spermatocytes and step 1-2 spermatids were the most susceptible cell stages to nitrobenzene and were observed forming into giant cells as early as 3 days after treatment. However, repair was substantial by 3 weeks after treatment and by days 76-100, the rate of sperm output reached 78% of the control group. By 100 days after treatment, there was greater than 90...Continue Reading

References

Sep 1, 1978·Journal of Reproduction and Fertility·G W RobbG J Killian
Jan 1, 1975·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·W J KortI MacDicken
Jan 1, 1988·Toxicology and Applied Pharmacology·D M BlackburnP M Foster
May 1, 1987·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·D E DoddG Kimmerle
Dec 1, 1985·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·W F BlazakB E Stewart
Nov 20, 1952·Annals of the New York Academy of Sciences·C P LEBLOND, Y CLERMONT
Jan 1, 1987·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P M FosterM S Prout

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Citations

Oct 27, 2001·BJU International·K AydosA Ergün
Mar 29, 2000·Archives of Environmental Health·R C Anderson, J H Anderson

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