PMID: 9537369Apr 16, 1998Paper

The Rhizobium etli rpoN locus: DNA sequence analysis and phenotypical characterization of rpoN, ptsN, and ptsA mutants

Journal of Bacteriology
J MichielsJ Vanderleyden

Abstract

The rpoN region of Rhizobium etli was isolated by using the Bradyrhizobium japonicum rpoN1 gene as a probe. Nucleotide sequence analysis of a 5,600-bp DNA fragment of this region revealed the presence of four complete open reading frames (ORFs), ORF258, rpoN, ORF191, and ptsN, coding for proteins of 258, 520, 191, and 154 amino acids, respectively. The gene product of ORF258 is homologous to members of the ATP-binding cassette-type permeases. ORF191 and ptsN are homologous to conserved ORFs found downstream from rpoN genes in other bacterial species. Unlike in most other microorganisms, rpoN and ORF191 are separated by approximately 1.6 kb. The R. etli rpoN gene was shown to control in free-living conditions the production of melanin, the activation of nifH, and the metabolism of C4-dicarboxylic acids and several nitrogen sources (ammonium, nitrate, alanine, and serine). Expression of the rpoN gene was negatively autoregulated and occurred independently of the nitrogen source. Inactivation of the ptsN gene resulted in a decrease of melanin synthesis and nifH expression. In a search for additional genes controlling the synthesis of melanin, an R. etli mutant carrying a Tn5 insertion in ptsA, a gene homologous to the Escherichia ...Continue Reading

References

Jun 11, 1992·Protein Science : a Publication of the Protein Society·J ReizerG R Jacobson
Sep 1, 1992·Molecular & General Genetics : MGG·E J PatriarcaM Iaccarino
Jun 1, 1992·Journal of Bacteriology·M LonettoC A Gross
Sep 1, 1991·Microbiological Reviews·J Collado-VidesJ D Gralla
Oct 1, 1990·Molecular Microbiology·M Chiurazzi, M Iaccarino
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S Sasse-Dwight, J D Gralla
Jun 1, 1987·Journal of Bacteriology·C W RonsonF M Ausubel
Jan 1, 1988·Annual Review of Biochemistry·J D Helmann, M J Chamberlin
Jan 1, 1984·Molecular & General Genetics : MGG·I Castaño, F Bastarrachea
May 1, 1984·Cell·F M Ausubel
Sep 15, 1995·Genes & Development·Y TintutJ D Gralla
Jan 15, 1994·FEMS Microbiology Letters·T KöhlerS Harayama
Nov 1, 1995·Molecular & General Genetics : MGG·I D'hoogheJ Vanderleyden
Aug 1, 1997·Journal of Bacteriology·R S Janakiraman, Y V Brun

❮ Previous
Next ❯

Citations

Nov 18, 1998·FEMS Microbiology Reviews·M M Wösten
Sep 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·C XiJ Michiels
Mar 30, 2001·Molecular Plant-microbe Interactions : MPMI·B DombrechtJ Michiels
May 15, 2002·FEMS Microbiology Letters·Susana Castro-SowinskiYaacov Okon
Sep 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·O Mario AguilarEitel Peltzer
Nov 5, 2005·Applied and Environmental Microbiology·Diana Hernández-RomeroAntonio Sanchez-Amat
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Mar 31, 2007·Microbial Ecology·Kristien BraekenJan Michiels
Apr 15, 2014·Applied Microbiology and Biotechnology·Shanjun GaoJan Michiels
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jessica B Warner, Juke S Lolkema
Oct 19, 1999·DNA Sequence : the Journal of DNA Sequencing and Mapping·J MichielsJ Vanderleyden
Mar 10, 2004·Applied and Environmental Microbiology·Martine MorisJan Michiels
Nov 25, 2004·Environmental Microbiology·V A P Martins Dos SantosK N Timmis
Oct 26, 2001·The Journal of Biological Chemistry·Ruth DanielsJan Michiels
Jul 14, 2000·Journal of Bacteriology·M BuckJ D Gralla
Jul 21, 2005·Journal of Bacteriology·Martine MorisJan Michiels
Mar 7, 2007·Molecular Genetics and Genomics : MGG·Thirunavukkarasu NagarajanAnil Kumar Tripathi
Mar 24, 2005·Molecular Plant-microbe Interactions : MPMI·Maria Péchy-TarrChristoph Keel
Sep 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ruth DanielsJan Michiels
May 11, 2010·Applied and Environmental Microbiology·Emmanuel SalazarSergio Encarnación
Jun 6, 2007·Infection and Immunity·Christa H Chatfield, Nicholas P Cianciotto
Oct 24, 2007·Journal of Applied Microbiology·U S GautamS K Das
May 5, 2007·The Journal of Biological Chemistry·Katharina Pflüger, Víctor de Lorenzo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.