The Rhizobium etli sigma70 (SigA) factor recognizes a lax consensus promoter

Nucleic Acids Research
M A Ramírez-RomeroGuillermo Dávila

Abstract

A collection of Rhizobium etli promoters was isolated from a genomic DNA library constructed in the promoter-trap vector pBBMCS53, by their ability to drive the expression of a gusA reporter gene. Thirty-seven clones were selected, and their transcriptional start-sites were determined. The upstream sequence of these 37 start-sites, and the sequences of seven previously identified promoters were compared. On the basis of sequence conservation and mutational analysis, a consensus sequence CTTGACN16-23TATNNT was obtained. In this consensus sequence, nine on of twelve bases are identical to the canonical Escherichia coli sigma70 promoter, however the R.etli promoters only contain 6.4 conserved bases on average. We show that the R.etli sigma factor SigA recognizes all R.etli promoters studied in this work, and that E.coli RpoD is incapable of recognizing them. The comparison of the predicted structure of SigA with the known structure of RpoD indicated that regions 2.4 and 4.2, responsible for promoter recognition, are different only by a single amino acid, whereas the region 1 of SigA contains 72 extra residues, suggesting that the differences contained in this region could be related to the lax promoter recognition of SigA.

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Citations

Aug 5, 2008·Journal of Bacteriology·Cristina BongiorniMarta Perego
Dec 26, 2006·Journal of Bacteriology·Maria HadjifrangiskouTheresa M Koehler
Feb 18, 2011·Journal of Bioinformatics and Computational Biology·Jianxiu Guo, Nini Rao
Jun 2, 2012·Virology Journal·Andrew M KropinskiRüdiger Schmitt
Sep 11, 2013·Plasmid·Angeles Pérez-Oseguera, Miguel A Cevallos
Apr 25, 2008·Plasmid·Miguel A CevallosRosa María Gutiérrez-Ríos
Feb 10, 2009·Microbiology·Jaime M Martínez-SalazarMiguel A Ramírez-Romero
Jul 29, 2016·Frontiers in Microbiology·Orlando SantillánGuillermo Dávila
Mar 8, 2011·Molecular Plant-microbe Interactions : MPMI·Jodie Box, K Dale Noel
Aug 7, 2009·Molecular Plant-microbe Interactions : MPMI·Allyson M MacleanTurlough M Finan
Mar 24, 2007·Microbiology·Cecilia I MugliaO Mario Aguilar
Jan 7, 2018·BMC Genomics·Angela KranzTino Polen
Dec 18, 2019·Nature Microbiology·Min-Hyung RyuChristopher A Voigt
Jul 6, 2021·Frontiers in Microbiology·Longxiang WangDeqiang Duanmu

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Datasets Mentioned

BETA
CP000133

Methods Mentioned

BETA
PCR
protein assay
electrophoretic

Software Mentioned

MultiAlin
WCONSENSUS
CLUSTALW

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