The Rhizopus oryzae secreted aspartic proteinase gene family: an analysis of gene expression.

Microbiology
Peter C Farley, Patrick A Sullivan

Abstract

Rhizopus oryzae was shown to possess a secreted aspartic proteinase gene family (sap) of at least four members (sap1-sap4). Within the family there was 77-87% identity at the nucleotide level and 76-92% identity at the amino acid level. Transcription of three members of this gene family (sap1-sap3) required an acidic medium (pH < 4.5) and either nitrogen or sulphur depression. Regulation was co-ordinate and hierarchical, with pH occupying the higher position in the hierarchy. Exogenous protein increased transcript levels, probably via the provision of metabolic intermediates rather than by direct induction of gene expression. sap4 was not expressed under these conditions. SAP1-SAP4 are predicted to have almost identical substrate-binding sites and therefore substrate specificity. It is proposed that sap1-sap3 exist to provide amplified expression of the secreted aspartic proteinase because protein, an important secondary nitrogen source for this fungus, requires extensive degradation to make its nitrogen available to the cell.

References

Dec 1, 1992·Journal of General Microbiology·P C Farley, L Ikasari
Aug 1, 1990·Molecular & General Genetics : MGG·T AshikariK Yano
May 15, 1987·Analytical Biochemistry·J LogemannL Willmitzer
Dec 1, 1988·European Journal of Biochemistry·C BetzelW Saenger
Jan 1, 1987·Mycopathologia·A Espinel-IngroffT M Kerkering
Aug 1, 1965·Canadian Journal of Microbiology·H L Wang, C W Hesseltine
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Jan 1, 1982·The International Journal of Biochemistry·M OhtsuruR Delaney
Jul 16, 1981·Nature·W H LiM Nei
Jan 1, 1994·Methods in Enzymology·A J Barrett
Jul 1, 1994·Molecular Microbiology·M MonodD Sanglard
Jul 15, 1993·FEMS Microbiology Letters·R S Chambers, P A Sullivan
Feb 1, 1997·Infection and Immunity·K FallonP Tamburini
Jan 1, 1997·Nucleic Acids Research·Y Van de PeerR De Wachter
Jan 1, 1997·Nucleic Acids Research·P De RijkR De Wachter
Feb 1, 1997·Fungal Genetics and Biology : FG & B·A LongacreB E Wright

❮ Previous
Next ❯

Citations

Aug 16, 2008·Antonie van Leeuwenhoek·Zhizhuang XiaoPeter C K Lau
Jan 21, 2004·Fungal Genetics and Biology : FG & B·Brad Hoffman, Colette Breuil
Sep 13, 2011·Journal of Agricultural and Food Chemistry·Jia-Shin LinChao-Hung Kao
Jan 25, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ashraf S IbrahimDimitrios P Kontoyiannis
Feb 12, 2009·Mycoses·Suganthini KrishnanPranatharthi H Chandrasekar
Nov 28, 2002·International Journal of Medical Microbiology : IJMM·Michel MonodOlivier Jousson
Aug 3, 2011·Current Opinion in Microbiology·Ashraf S Ibrahim
Jun 17, 2016·The Annals of Pharmacotherapy·Treavor T RileyDavey P Legendre
Sep 3, 2009·Journal of Agricultural and Food Chemistry·Chun-Chang ChenWen-Hwei Hsu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.