The Rho Termination Factor of Clostridium botulinum Contains a Prion-Like Domain with a Highly Amyloidogenic Core

Frontiers in Microbiology
Irantzu PallarèsSalvador Ventura

Abstract

Prion-like proteins can switch between a soluble intrinsically disordered conformation and a highly ordered amyloid assembly. This conformational promiscuity is encoded in specific sequence regions, known as prion domains (PrDs). Prions are best known as the causative factors of neurological diseases in mammals. However, bioinformatics analyses reveal that proteins bearing PrDs are present in all kingdoms of life, including bacteria, thus supporting the idea that they serve conserved beneficial cellular functions. Despite the proportion of predicted prion-like proteins in bacterial proteomes is generally low, pathogenic species seem to have a higher prionic load, suggesting that these malleable proteins may favor pathogenic traits. In the present work, we performed a stringent computational analysis of the Clostridium botulinum pathogen proteome in the search for prion-like proteins. A total of 54 candidates were predicted for this anaerobic bacterium, including the transcription termination Rho factor. This RNA-binding protein has been shown to play a crucial role in bacterial adaptation to changing environments. We show here that the predicted disordered PrD domain of this RNA-binding protein contains an inner, highly polar, ...Continue Reading

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Citations

May 8, 2018·International Journal of Molecular Sciences·Jordi PujolsSalvador Ventura
Nov 3, 2017·Current Genetics·Elena Bidnenko, Vladimir Bidnenko
May 11, 2020·Journal of Molecular Evolution·Jamie D Dixson, Rajeev K Azad
Mar 9, 2017·Expert Review of Proteomics·Cristina BatlleSalvador Ventura
Apr 20, 2017·Frontiers in Microbiology·Laura Molina-GarcíaRafael Giraldo
May 17, 2017·Frontiers in Microbiology·Alexander Gill
Sep 25, 2017·Scientific Reports·Cristina BatlleSalvador Ventura
Apr 12, 2019·Frontiers in Physiology·Valentin IglesiasSalvador Ventura
Jan 16, 2019·BMC Bioinformatics·Valentin IglesiasSalvador Ventura
Jan 14, 2017·Science·Andy H Yuan, Ann Hochschild
Aug 23, 2018·Frontiers in Microbiology·Irantzu PallarèsSalvador Ventura
Sep 7, 2018·Frontiers in Microbiology·Gundeep KaurKrishan G Thakur
Oct 4, 2020·International Journal of Molecular Sciences·Anastasiia O KosolapovaAnton A Nizhnikov

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Methods Mentioned

BETA
light scattering
glycotransferase
Fluorescence
fluorescence microscopy
light

Software Mentioned

GAGAfactor
pWALTZ
PeakFit
TANGO
FoldIndex
PAPA
PrionScan
FoldAmyloid
Systat
AGGRESCAN

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