The Rho3 and Rho4 small GTPases interact functionally with Wsc1p, a cell surface sensor of the protein kinase C cell-integrity pathway in Saccharomyces cerevisiae

Microbiology
Helder FernandesM Crouzet

Abstract

Rgd1, a GTPase-activating protein, is the only known negative regulator of the Rho3 and Rho4 small GTPases in the yeast Saccharomyces cerevisiae. Rho3p and Rho4p are involved in regulating cell polarity by controlling polarized exocytosis. Co-inactivation of RGD1 and WSC1, which is a cell wall sensor-encoding gene, is lethal. Another plasma membrane sensor, Mid2p, is known to rescue the rgd1Deltawsc1Delta synthetic lethality. It has been proposed that Wsc1p and Mid2p act upstream of the protein kinase C (PKC) pathway to function as mechanosensors of cell wall stress. Analysis of the synthetic lethal phenomenon revealed that production of activated Rho3p and Rho4p leads to lethality in wsc1Delta cells. Inactivation of RHO3 or RHO4 was able to rescue the rgd1Deltawsc1Delta synthetic lethality, supporting the idea that the accumulation of GTP-bound Rho proteins, following loss of Rgd1p, is detrimental if the Wsc1 sensor is absent. In contrast, the genetic interaction between RGD1 and MID2 was not due to an accumulation of GTP-bound Rho proteins. It was proposed that simultaneous inactivation of RGD1 and WSC1 constitutively activates the PKC-mitogen-activated protein kinase (MAP kinase) pathway. Moreover, it was shown that the acti...Continue Reading

References

Jan 1, 1975·Methods in Cell Biology·A H Rose
Feb 1, 1991·Molecular and Cellular Biology·S SilveR Haguenauer-Tsapis
Apr 19, 1996·The Journal of Biological Chemistry·Y KamadaD E Levin
Oct 6, 1997·Genes & Development·L Van Aelst, C D'Souza-Schorey
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·J VernaR Ballester
Oct 14, 1998·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·C BartheM Crouzet
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·M C GustinK Davenport
May 18, 1999·Molecular and Cellular Biology·M RajavelD E Levin
Dec 26, 2001·EMBO Reports·Eric Fernandez-BellotChristophe Cullin
Aug 13, 2002·The Journal of Biological Chemistry·Jordi TorresMaria Angeles De La Torre-Ruiz
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Jun 18, 2003·The Journal of Cell Biology·Yuqing DongAnthony Bretscher

❮ Previous
Next ❯

Citations

Oct 11, 2008·The Journal of Biological Chemistry·Valérie Prouzet-MauléonFrançois Doignon
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Hay-Oak Park, Erfei Bi
Mar 5, 2013·Biochemical and Biophysical Research Communications·Aurélie VieillemardDidier Thoraval
Jan 11, 2011·Biochemical and Biophysical Research Communications·Benoît OdaertMichel Hugues
Jul 20, 2010·Yeast·Rosaura Rodicio, Jürgen J Heinisch
Jan 20, 2015·Molecular Microbiology·Hans-Peter SchmitzJürgen J Heinisch

❮ Previous
Next ❯

Related Concepts

Related Feeds