The Rhodobacter capsulatus hupSLC promoter: identification of cis-regulatory elements and of trans-activating factors involved in H2 activation of hupSLC transcription

Molecular Microbiology
B ToussaintP M Vignais

Abstract

The [NiFe]hydrogenase of the photosynthetic bacterium Rhodobacter capsulatus is encoded by the structural hupSLC operon, the expression of which is induced by H2. H2 activation was no longer observable in chromosomal hupR mutants, an indication that HupR is implicated directly in the activation by H2 of hupS gene expression. The transcriptional start site of the hupS promoter, determined by primer extension mapping, was located 55 nucleotides upstream from the translational start codon of the hupS gene. Regulatory sequences were identified by serial 5' deletions of the 300bp hupS promoter-regulatory region (phupS) and phupS-lacZ translational fusions. Cis-regulatory sequences capable of interacting with two transcription factors, IHF and HupR, a response regulator of the NtrC subfamily, were studied by electrophoretic mobility shift assays (EMSAs). The R. capsulatus IHF and HupR proteins were overexpressed in Escherichia coli and purified by affinity chromatography. IHF binds to a site, 5'-TCACACACCATTG, centred at -87 nt from the transcription start site. The HupR protein binds to one site within the -162 to -152 nt region, which contains the palindromic sequence 5'-TTG-R5-CAA. By the use of 5' deletions and site-directed muta...Continue Reading

Citations

Jan 1, 1994·Archives of Microbiology·P M Vignais, B Toussaint
Jul 17, 1998·Current Opinion in Chemical Biology·S W Ragsdale
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Sylvie ElsenCarl E Bauer
Feb 17, 2007·Nature Reviews. Genetics·Gregory A Wray
Jan 26, 2005·Biochemical Society Transactions·P M VignaisA Colbeau
Aug 29, 2001·FEMS Microbiology Reviews·P M VignaisJ Meyer
Oct 12, 2007·Chemical Reviews·Paulette M Vignais, Bernard Billoud
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·S C BakerR J van Spanning
Apr 25, 2006·Journal of Molecular Biology·Karen M DaviesCatherine Vénien-Bryan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.