The ribonucleotidyl transferase USIP-1 acts with SART3 to promote U6 snRNA recycling

Nucleic Acids Research
Stefan RüeggerHelge Großhans

Abstract

The spliceosome is a large molecular machine that serves to remove the intervening sequences that are present in most eukaryotic pre-mRNAs. At its core are five small nuclear ribonucleoprotein complexes, the U1, U2, U4, U5 and U6 snRNPs, which undergo dynamic rearrangements during splicing. Their reutilization for subsequent rounds of splicing requires reversion to their original configurations, but little is known about this process. Here, we show that ZK863.4/USIP-1 (U Six snRNA-Interacting Protein-1) is a ribonucleotidyl transferase that promotes accumulation of the Caenorhabditis elegans U6 snRNA. Endogenous USIP-1-U6 snRNA complexes lack the Lsm proteins that constitute the protein core of the U6 snRNP, but contain the U6 snRNP recycling factor SART3/B0035.12. Furthermore, co-immunoprecipitation experiments suggest that SART3 but not USIP-1 occurs also in a separate complex containing both the U4 and U6 snRNPs. Based on this evidence, genetic interaction between usip-1 and sart-3, and the apparent dissociation of Lsm proteins from the U6 snRNA during spliceosome activation, we propose that USIP-1 functions upstream of SART3 to promote U6 snRNA recycling.

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Citations

Feb 21, 2016·Life Sciences·Amanda WhitmillJohnny J He
Jan 27, 2016·Nucleic Acids Research·Gabrielle HaasSébastien Pfeffer
Dec 4, 2015·International Journal of Molecular Sciences·Aldine R AmielEric Röttinger
Sep 16, 2016·RNA Biology·David Staněk
Jan 24, 2017·Molecular Cell·Florian AeschimannHelge Großhans
Jan 26, 2018·RNA·Allison L DidychukDavid A Brow
May 1, 2020·Cold Spring Harbor Symposia on Quantitative Biology·Anna MattoutSusan M Gasser
Apr 17, 2019·Nature Methods·Melanie A PrestonMarvin Wickens
Oct 7, 2017·Wiley Interdisciplinary Reviews. RNA·Caroline De AlmeidaDominique Gagliardi
Mar 27, 2019·Life Science Alliance·Florian AeschimannHelge Großhans
Nov 5, 2020·Wiley Interdisciplinary Reviews. RNA·Vladyslava Liudkovska, Andrzej Dziembowski

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Methods Mentioned

BETA
deubiquitination
transgenic
electrophoresis
PCR
Fluorescence
co-immunoprecipitation
co-IPs
co-IP
Assay
Transferase assay

Software Mentioned

MosSCI
AxioVision SE64
Gibson Assembly
ImageJ
Mascot

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