PMID: 7519982Jul 15, 1994Paper

The ribosomal protein S8 from Thermus thermophilus VK1. Sequencing of the gene, overexpression of the protein in Escherichia coli and interaction with rRNA

European Journal of Biochemistry
V VysotskayaB Ehresmann

Abstract

The gene of the ribosomal protein S8 from Thermus thermophilus VK1 has been isolated from a genomic library by hybridization of an oligonucleotide coding for the N-terminal amino acid sequence of the protein, amplified by PCR and sequenced. Nucleotide sequence reveals an open reading frame coding for a protein of 138 amino acid residues (M(r) 15,839). The codon usage shows that 94% of the codons possess G or C in the third position, and agrees with the preferential usage of codons of high G+C content in the bacteria of the genus Thermus. The amino acid sequence of the protein shows 48% identity with the protein from Escherichia coli. Ribosomal protein S8 from T. thermophilus has been expressed in E. coli under the control of the T7 promoter and purified to homogeneity by heat treatment of the extract followed by cation-exchange chromatography. Conditions were defined in which T. thermophilus protein S8 binds specifically an homologous 16S rRNA fragment containing the putative S8 binding site with an apparent association constant of 5 x 10(7) M-1. The overexpressed protein binds the rRNA with the same affinity as that extracted from T. thermophilus, indicating that the thermophilic protein is correctly folded in E. coli. The spe...Continue Reading

References

Jul 26, 1979·Biochimica Et Biophysica Acta·R A Zimmermann, K Singh-Bergmann
Jul 1, 1991·Biochimie·M M YusupovA S Spirin
May 8, 1991·European Journal of Biochemistry·O JahnV A Erdmann
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L MattheakisM Nomura
Aug 25, 1988·Nucleic Acids Research·N V MurzinaN I Matvienko
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Jan 1, 1969·Cold Spring Harbor Symposia on Quantitative Biology·M NomuraC V Lowry
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J L YatesM Nomura
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·N BrotH Weissbach
Feb 1, 1993·Journal of Protein Chemistry·J ReinboltB Ehresmann

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Citations

Aug 15, 1998·Biological Chemistry·S V NikonovM B Garber
Mar 17, 2018·Microbiology Spectrum·Michelle M Meyer

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