PMID: 9547137Apr 18, 1998Paper

The ribosomal RNA gene region of Nosema apis (Microspora): DNA sequence for small and large subunit rRNA genes and evidence of a large tandem repeat unit size

Journal of Invertebrate Pathology
H S Gatehouse, L A Malone

Abstract

The ribosomal RNA (rRNA) gene region of the microsporidium, Nosema apis, has been examined. A new method for extracting microsporidian genomic DNA from infected host tissue is described. Complete DNA sequence data are presented for the small subunit gene (1242 bp), the internal transcribed space (33 bp), and the large subunit gene (2481 bp to a putative termination point). This is the first time that the complete large subunit rRNA gene has been published for any microsporidian species. DNA sequence is also presented for the regions flanking the 5' end of the small subunit gene and the 3' end of the large subunit gene. The intergenic spacer is shown to be heterogeneous, showing variation in sequence and restriction sites rather than length and containing sequence repeats, which are a characteristic feature of intergenic spacers. The rRNA gene region of N. apis is shown to occur in a head-to-tail, tandemly repeated manner, as in other eukaryotes. This repeat unit is shown to be approximately 18 kb in length. The nucleotide sequence presented has been submitted to the Genbank database under the accession number U97150.

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Citations

Apr 27, 2007·Nucleic Acids Research·Annette W Coleman
Jun 21, 2003·Proceedings. Biological Sciences·Calum MacNeilAlison M Dunn
Mar 17, 2010·Applied and Environmental Microbiology·Sebastian GisderElke Genersch
Mar 31, 2007·BMC Evolutionary Biology·Joseph E Ironside
Feb 5, 2014·Diseases of Aquatic Organisms·Mona SalehSaleh Al-Quraishy
Sep 2, 2008·Journal of Insect Physiology·Noah Wilson-RichPhilip T Starks
Feb 16, 2007·The Journal of Eukaryotic Microbiology·Elaine M O'MahonyRobert J Paxton
Feb 16, 2007·The Journal of Eukaryotic Microbiology·Dominik Refardt, Laurence Mouton
Feb 7, 2008·The Journal of Eukaryotic Microbiology·George Kyei-PokuKees van Frankenhuyzen
Aug 7, 2008·The Journal of Eukaryotic Microbiology·Joseph E IronsideJennifer Rock
Nov 26, 2009·FEMS Microbiology Reviews·Ana Lilia Torres-MachorroImelda López-Villaseñor
Jul 22, 2008·Journal of Invertebrate Pathology·Handeng LiuZeyang Zhou
Nov 26, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Lukas KrebesRalf Bastrop
Jul 16, 2013·International Journal for Parasitology·Nicolas O RodeThomas Lenormand
Oct 21, 2016·BMC Evolutionary Biology·Soledad SagastumeNuno Henriques-Gil
Dec 4, 2014·Revista Da Sociedade Brasileira De Medicina Tropical·Isabel Maria Vicente Guedes de CarvalhoCarlos José Pereira da Cunha de Araujo-Coutinho
Jul 19, 2005·International Journal for Parasitology·H Elizabeth McClymontJudith E Smith
Jun 2, 2006·Journal of Invertebrate Pathology·Jay D Evans
Mar 10, 2017·The Journal of Eukaryotic Microbiology·Longxin ChenLong Zhang
Mar 27, 2018·Environmental Microbiology·Raquel Martín-HernándezMariano Higes
Feb 11, 2005·The Journal of Eukaryotic Microbiology·Shu-Jen TsaiChung-Hsiung Wang

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