The ribosomal stalk protein is crucial for the action of the conserved ATPase ABCE1

Nucleic Acids Research
Hirotatsu ImaiToshio Uchiumi

Abstract

The ATP-binding cassette (ABC) protein ABCE1 is an essential factor in ribosome recycling during translation. However, the detailed mechanochemistry of its recruitment to the ribosome, ATPase activation and subunit dissociation remain to be elucidated. Here, we show that the ribosomal stalk protein, which is known to participate in the actions of translational GTPase factors, plays an important role in these events. Biochemical and crystal structural data indicate that the conserved hydrophobic amino acid residues at the C-terminus of the archaeal stalk protein aP1 binds to the nucleotide-binding domain 1 (NBD1) of aABCE1, and that this binding is crucial for ATPase activation of aABCE1 on the ribosome. The functional role of the stalk•ABCE1 interaction in ATPase activation and the subunit dissociation is also investigated using mutagenesis in a yeast system. The data demonstrate that the ribosomal stalk protein likely participates in efficient actions of both archaeal and eukaryotic ABCE1 in ribosome recycling. The results also show that the stalk protein has a role in the function of ATPase as well as GTPase factors in translation.

References

Jun 5, 1987·Journal of Molecular Biology·M Leijonmarck, A Liljas
Aug 29, 2002·The Journal of Biological Chemistry·Toshio UchiumiAkira Hachimori
Oct 9, 2002·Biochemistry·Dagmar MohrMarina V Rodnina
Oct 7, 2003·Molecular Cell·Jue ChenFlorante A Quiocho
Feb 13, 2004·The Journal of Biological Chemistry·Eduard V BocharovAlexander S Arseniev
Jun 11, 2004·Annual Review of Biochemistry·Lee D Kapp, Jon R Lorsch
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Sep 7, 2007·The Journal of Biological Chemistry·Yasushi MakiToshio Uchiumi
Oct 5, 2007·Methods in Enzymology·Byung-Sik Shin, Thomas E Dever
Dec 21, 2007·Acta Crystallographica. Section D, Biological Crystallography·Fei LongGarib N Murshudov
Dec 28, 2007·The Journal of Biological Chemistry·Annette KarcherKarl-Peter Hopfner
Jul 10, 2008·Journal of Molecular Evolution·Przemysław GrelaMarek Tchórzewski
Feb 27, 2009·Current Opinion in Cell Biology·Marina V Rodnina, Wolfgang Wintermeyer
Dec 17, 2009·The Journal of Biological Chemistry·Takao NaganumaIsao Tanaka
Feb 4, 2010·Molecular Cell·Andrey V PisarevTatyana V Pestova
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Mar 30, 2010·Nature Chemical Biology·Nina ClementiNorbert Polacek
Nov 6, 2010·Science·Rebecca M VoorheesV Ramakrishnan
Feb 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Dominik BarthelmeRobert Tampé
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Garib N MurshudovAlexei A Vagin
Dec 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christopher J Shoemaker, Rachel Green
Feb 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Naoko NomuraToshio Uchiumi
Oct 4, 2012·Current Opinion in Structural Biology·Sibylle FranckenbergRoland Beckmann
Dec 26, 2012·Trends in Biochemical Sciences·Elina Nürenberg, Robert Tampé
Jun 3, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hirotatsu ImaiToshio Uchiumi
Aug 8, 2015·Nature·Alan BrownV Ramakrishnan
Dec 23, 2016·Nature Communications·Lucas D SerdarKristian E Baker
Apr 4, 2017·Nature Structural & Molecular Biology·André HeuerRobert Tampé

❮ Previous
Next ❯

Citations

Oct 31, 2019·Biological Chemistry·Elina Nürenberg-Goloub, Robert Tampé
Feb 26, 2019·Microbial Biotechnology·Grégory BoëlAntoine Danchin
Dec 9, 2020·Proceedings of the National Academy of Sciences of the United States of America·Hirotatsu ImaiNoriyuki Kodera
Dec 9, 2020·The EMBO Journal·Hanna KratzatRoland Beckmann

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPase
PCR
size-extraction chromatography
electrophoresis
footprinting
pull-down
Fluorescence
density gradient centrifugation
X-ray

Software Mentioned

BALBES
XDS
GraphPad Prism
GraphPad
COOT
REFMAC5

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.