PMID: 7003539Sep 11, 1980

The ribosome binding sites recognized by E. coli ribosomes have regions with signal character in both the leader and protein coding segments

Nucleic Acids Research
G F SchererD J Morré

Abstract

Oligonucleotide analysis, by a novel computerized procedure, was first applied to determine the sequence of an ideal E. coli promoter (Scherer et al., Nucl. Acids Res. 1978, 5:3759-3773) and has now been used to obtain the sequence of nucleotides that should be present in a messenger RNA for optimum binding to the E. coli ribosome. This sequence is: UU.UUAAAAAUUAAGGAGGUAUAUUAUGAAAAAAAUUAAAAAACUCAA AA U A AUA A CUC G. Comparison of this sequence with each of the 68 ribosome binding site sequences used to generate it shows a preference rather than an absolute requirement for a specific base in any given position. The preference for certain bases persists along the whole length of the RNA within the ribosome binding domain even though nearly half of that length includes translated codons. Thus messages without leader sequences (like lambda CI mRNA) can still have some affinity for the ribosome. Part of the model sequence is complementary to the 3'end of 16S rRNA.

References

Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·N Lee, J Carbon
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J J DunnF W Studier
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D BelinR H Epstein
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J F Gardner
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R A BaanL Bosch
Aug 25, 1977·Journal of Molecular Biology·J A Steitz, D A Steege
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D A Steege
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R M PirtleM Inouye
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J G Sutcliffe
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G ZurawskiC Yanofsky
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W M Barnes
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T M RobertsM Ptashne
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L E PostP P Dennis
May 15, 1979·Journal of Molecular Biology·E Selker, C Yanofsky
Apr 1, 1979·Nucleic Acids Research·V Pirrotta
Nov 16, 1978·Nature·G N GodsonJ C Fiddes
Oct 25, 1978·Journal of Molecular Biology·F SangerM Smith
Dec 1, 1978·Nucleic Acids Research·E BeckM Takanami
May 15, 1976·Journal of Molecular Biology·C SquiresC Yanofsky
Jul 24, 1975·Nature·W FiersM Van Montagu
Aug 25, 1977·Journal of Molecular Biology·J A Steitz, R A Bryan
Aug 25, 1977·Journal of Molecular Biology·D A Steege
Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·K Isono, S Isono
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T Platt, C Yanofsky
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A E Dahlberg, J E Dahlberg
Dec 5, 1974·Journal of Molecular Biology·G PieczenikH D Robertson
Mar 22, 1972·Nature: New Biology·J A Steitz
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·W G SpiegelmanH Eisen
Jun 7, 1972·Nature: New Biology·H WeberA Porter
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J G FilesJ H Miller
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Jun 14, 1974·Nature·N Maizels
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R E MussoS M Weissman
Jun 5, 1970·Biochemical and Biophysical Research Communications·S L GuptaS M Weissman
Sep 15, 1971·Nature: New Biology·D H StaplesC Weissmann
Sep 15, 1971·Nature: New Biology·D H Staples, J Hindley
Dec 6, 1969·Nature·J Hindley, D H Staples

Citations

Nov 1, 1988·Molecular & General Genetics : MGG·M SuissaA B Oppenheim
May 1, 1987·Molecular & General Genetics : MGG·S ShinedlingL Gold
Jan 1, 1982·Molecular & General Genetics : MGG·H Bedouelle, M Hofnung
Jan 1, 1984·Molecular & General Genetics : MGG·H OhsawaC O Gualerzi
Feb 1, 1988·Journal of Bioenergetics and Biomembranes·J E McCarthy
Feb 1, 1991·Applied Biochemistry and Biotechnology·M KobayashiH Yukawa
Nov 1, 1994·Trends in Genetics : TIG·J E McCarthy, R Brimacombe
May 1, 1995·The International Journal of Biochemistry & Cell Biology·R AlexandrovaI G Ivanov
Jul 31, 2003·Molecular Cell·Heath D MurrayRichard L Gourse
Apr 1, 1997·The International Journal of Biochemistry & Cell Biology·I G IvanovM G AbouHaidar
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M SchwartzM Débarbouillé
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G JayE Jay
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K F WertmanD W Mount
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J C LacalR Crowl
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W MandeckiJ L Fox
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W F JacobA E Dahlberg
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R A CalogeroClaudio O Gualerzi
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M H de Smit, J van Duin
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A La TeanaClaudio O Gualerzi
Jan 1, 1984·Biotechnology & Genetic Engineering Reviews·M D Winther, G Dougan
Apr 1, 1990·AIDS Research and Human Retroviruses·R L GarlickC S Tomich
May 11, 1982·Nucleic Acids Research·Gary D StormoL Gold
Jul 11, 1983·Nucleic Acids Research·M BensimhonC Reiss
Jan 11, 1984·Nucleic Acids Research·M Johnsen
Jan 11, 1985·Nucleic Acids Research·A S Kolaskar, B V Reddy
Jan 11, 1991·Nucleic Acids Research·I V BoniN V Tzareva
Mar 25, 1994·Nucleic Acids Research·P H Pouwels, J A Leunissen
Jan 24, 2007·Molecular Microbiology·Jae-Sung YuD A Steege
Dec 20, 1985·Journal of Molecular Biology·K C Cone, D A Steege
Jan 20, 1990·Journal of Molecular Biology·C PortierM Grunberg-Manago
Apr 20, 1992·Journal of Molecular Biology·M Ivey-Hoyle, D A Steege
Apr 5, 1985·Journal of Molecular Biology·M DreyfusS Busby
Nov 15, 1982·Journal of Molecular Biology·H BedouelleM Rosenberg
Aug 7, 1992·Cell·C S Shean, M E Gottesman
Apr 1, 1986·Medicinal Research Reviews·S P Adams, G R Galluppi
Sep 1, 1987·Scandinavian Journal of Immunology·P BillP A Peterson
Jan 1, 1985·CRC Critical Reviews in Biochemistry·H P DriessenH Bloemendal
Jun 26, 1990·Biochemistry·C O Gualerzi, C L Pon
Jun 3, 2017·Nucleic Acids Research·He ChengguangClaudio O Gualerzi
Jul 1, 1990·Molecular Microbiology·N Jacques, M Dreyfus
Mar 4, 2020·Applied Microbiology and Biotechnology·Tomo Kondo, Shigehiko Yumura
Jul 22, 2014·Cold Spring Harbor Symposia on Quantitative Biology·Gary D Stormo

Related Concepts

Alkalescens-Dispar Group
RNA Conformation
Oligoribonucleotides
Operon
Ribosomes
15S RNA
Protein Biosynthesis

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.